TY  - JOUR
A1  - Sivanesan, Arumugam
A1  - Kalaivani, Govindasamy
A1  - Fischer, Anna
A1  - Stiba, Konstanze
A1  - Leimkühler, Silke
A1  - Weidinger, Inez M.
T1  - Complementary surface-enhanced resonance raman Spectroscopic Biodetection of mixed protein solutions by Chitosan- and Silica-Coated Plasmon-Tuned Silver Nanoparticles
JF  - Analytical chemistry
N2  - Silver nanoparticles with identical plasmonic properties but different surface functionalities are synthesized and tested as chemically selective surface-enhanced resonance Raman (SERR) amplifiers in a two-component protein solution. The surface plasmon resonances of the particles are tuned to 413 nm to match the molecular resonance of protein heme cofactors. Biocompatible functionalization of the nanoparticles with a thin film of chitosan yields selective SERR enhancement of the anionic protein cytochrome b(5), whereas functionalization with SiO2 amplifies only the spectra of the cationic protein cytochrome c. As a result, subsequent addition of the two differently functionalized particles yields complementary information on the same mixed protein sample solution. Finally, the applicability of chitosan-coated Ag nanoparticles for protein separation was tested by in situ resonance Raman spectroscopy.
Y1  - 2012
U6  - https://doi.org/10.1021/ac301001a
SN  - 0003-2700
VL  - 84
IS  - 13
SP  - 5759
EP  - 5764
PB  - American Chemical Society
CY  - Washington
ER  -