TY  - JOUR
A1  - Robaina-Estevez, Semidan
A1  - Daloso, Danilo M.
A1  - Zhang, Youjun
A1  - Fernie, Alisdair R.
A1  - Nikoloski, Zoran
T1  - Resolving the central metabolism of Arabidopsis guard cells
JF  - Scientific reports
N2  - Photosynthesis and water use efficiency, key factors affecting plant growth, are directly controlled by microscopic and adjustable pores in the leaf-the stomata. The size of the pores is modulated by the guard cells, which rely on molecular mechanisms to sense and respond to environmental changes. It has been shown that the physiology of mesophyll and guard cells differs substantially. However, the implications of these differences to metabolism at a genome-scale level remain unclear. Here, we used constraint-based modeling to predict the differences in metabolic fluxes between the mesophyll and guard cells of Arabidopsis thaliana by exploring the space of fluxes that are most concordant to cell-type-specific transcript profiles. An independent C-13-labeling experiment using isolated mesophyll and guard cells was conducted and provided support for our predictions about the role of the Calvin-Benson cycle in sucrose synthesis in guard cells. The combination of in silico with in vivo analyses indicated that guard cells have higher anaplerotic CO2 fixation via phosphoenolpyruvate carboxylase, which was demonstrated to be an important source of malate. Beyond highlighting the metabolic differences between mesophyll and guard cells, our findings can be used in future integrated modeling of multicellular plant systems and their engineering towards improved growth.
Y1  - 2017
U6  - https://doi.org/10.1038/s41598-017-07132-9
SN  - 2045-2322
VL  - 7
SP  - 1913
EP  - 1932
PB  - Nature Publ. Group
CY  - London
ER  -