TY  - GEN
A1  - Nikoloski, Zoran
A1  - van Dongen, Joost T.
T1  - Modeling alternatives for interpreting the change in oxygen-consumption rates during hypoxic conditions
T2  - New phytologist : international journal of plant science
KW  - hypoxia
KW  - modeling
KW  - regulation
KW  - respiration
KW  - roots
Y1  - 2011
U6  - https://doi.org/10.1111/j.1469-8137.2011.03674.x
SN  - 0028-646X
VL  - 190
IS  - 2
SP  - 273
EP  - 276
PB  - Wiley-Blackwell
CY  - Malden
ER  - 
TY  - JOUR
A1  - Araujo, Wagner L.
A1  - Nunes-Nesi, Adriano
A1  - Nikoloski, Zoran
A1  - Sweetlove, Lee J.
A1  - Fernie, Alisdair R.
T1  - Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues
JF  - Plant, cell & environment : cell physiology, whole-plant physiology, community physiology
N2  - The tricarboxylic acid (TCA) cycle is a crucial component of respiratory metabolism in both photosynthetic and heterotrophic plant organs. All of the major genes of the tomato TCA cycle have been cloned recently, allowing the generation of a suite of transgenic plants in which the majority of the enzymes in the pathway are progressively decreased. Investigations of these plants have provided an almost complete view of the distribution of control in this important pathway. Our studies suggest that citrate synthase, aconitase, isocitrate dehydrogenase, succinyl CoA ligase, succinate dehydrogenase, fumarase and malate dehydrogenase have control coefficients flux for respiration of -0.4, 0.964, -0.123, 0.0008, 0.289, 0.601 and 1.76, respectively; while 2-oxoglutarate dehydrogenase is estimated to have a control coefficient of 0.786 in potato tubers. These results thus indicate that the control of this pathway is distributed among malate dehydrogenase, aconitase, fumarase, succinate dehydrogenase and 2-oxoglutarate dehydrogenase. The unusual distribution of control estimated here is consistent with specific non-cyclic flux mode and cytosolic bypasses that operate in illuminated leaves. These observations are discussed in the context of known regulatory properties of the enzymes and some illustrative examples of how the pathway responds to environmental change are given.
KW  - metabolic control analysis
KW  - metabolic regulation
KW  - respiration
KW  - Solanum lycopersicum (tomato)
KW  - TCA cycle
Y1  - 2012
U6  - https://doi.org/10.1111/j.1365-3040.2011.02332.x
SN  - 0140-7791
VL  - 35
IS  - 1
SP  - 1
EP  - 21
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Jose Clemente-Moreno, Maria
A1  - Omranian, Nooshin
A1  - Saez, Patricia
A1  - Maria Figueroa, Carlos
A1  - Del-Saz, Nestor
A1  - Elso, Mhartyn
A1  - Poblete, Leticia
A1  - Orf, Isabel
A1  - Cuadros-Inostroza, Alvaro
A1  - Cavieres, Lohengrin
A1  - Bravo, Leon
A1  - Fernie, Alisdair R.
A1  - Ribas-Carbo, Miquel
A1  - Flexas, Jaume
A1  - Nikoloski, Zoran
A1  - Brotman, Yariv
A1  - Gago, Jorge
T1  - Cytochrome respiration pathway and sulphur metabolism sustain stress tolerance to low temperature in the Antarctic species Colobanthus quitensis
JF  - New phytologist : international journal of plant science
N2  - Understanding the strategies employed by plant species that live in extreme environments offers the possibility to discover stress tolerance mechanisms. We studied the physiological, antioxidant and metabolic responses to three temperature conditions (4, 15, and 23 degrees C) of Colobanthus quitensis (CQ), one of the only two native vascular species in Antarctica. We also employed Dianthus chinensis (DC), to assess the effects of the treatments in a non-Antarctic species from the same family. Using fused LASSO modelling, we associated physiological and biochemical antioxidant responses with primary metabolism. This approach allowed us to highlight the metabolic pathways driving the response specific to CQ. Low temperature imposed dramatic reductions in photosynthesis (up to 88%) but not in respiration (sustaining rates of 3.0-4.2 mu mol CO2 m(-2) s(-1)) in CQ, and no change in the physiological stress parameters was found. Its notable antioxidant capacity and mitochondrial cytochrome respiratory activity (20 and two times higher than DC, respectively), which ensure ATP production even at low temperature, was significantly associated with sulphur-containing metabolites and polyamines. Our findings potentially open new biotechnological opportunities regarding the role of antioxidant compounds and respiratory mechanisms associated with sulphur metabolism in stress tolerance strategies to low temperature.
KW  - Antarctica
KW  - antioxidant capacity
KW  - low temperature
KW  - photosynthesis
KW  - respiration
KW  - stress tolerance
KW  - sulphur metabolism
Y1  - 2019
U6  - https://doi.org/10.1111/nph.16167
SN  - 0028-646X
SN  - 1469-8137
VL  - 225
IS  - 2
SP  - 754
EP  - 768
PB  - Wiley
CY  - Hoboken
ER  -