TY  - GEN
A1  - Hardy, John G.
A1  - Torres-Rendon, Jose Guillermo
A1  - Leal-Egaña, Aldo
A1  - Walther, Andreas
A1  - Schlaad, Helmut
A1  - Cölfen, Helmut
A1  - Scheibel, Thomas R.
T1  - Biomineralization of engineered spider silk protein-based composite materials for bone tissue engineering
N2  - Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 359 
KW  - spider silk
KW  - recombinant protein
KW  - biodegradable polymers
KW  - biomaterials
KW  - biomineralization
KW  - bone tissue engineering
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-400519
ER  - 
TY  - JOUR
A1  - Hardy, John G.
A1  - Torres-Rendon, Jose Guillermo
A1  - Leal-Egana, Aldo
A1  - Walther, Andreas
A1  - Schlaad, Helmut
A1  - Coelfen, Helmut
A1  - Scheibel, Thomas R.
T1  - Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
JF  - Materials
N2  - Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
KW  - spider silk
KW  - recombinant protein
KW  - biodegradable polymers
KW  - biomaterials
KW  - biomineralization
KW  - bone tissue engineering
Y1  - 2016
U6  - https://doi.org/10.3390/ma9070560
SN  - 1996-1944
VL  - 9
SP  - 93
EP  - 108
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Hardy, John G.
A1  - Bertin, Annabelle
A1  - Torres-Rendon, Jose Guillermo
A1  - Leal-Egana, Aldo
A1  - Humenik, Martin
A1  - Bauer, Felix
A1  - Walther, Andreas
A1  - Cölfen, Helmut
A1  - Schlaad, Helmut
A1  - Scheibel, Thomas R.
T1  - Facile photochemical modification of silk protein-based biomaterials
JF  - Macromolecular bioscience
N2  - Silk protein-based materials show promise for application as biomaterials for tissue engineering. The simple and rapid photochemical modification of silk protein-based materials composed of either Bombyx mori silkworm silk or engineered spider silk proteins (eADF4(C16)) is reported. Radicals formed on the silk-based materials initiate the polymerization of monomers (acrylic acid, methacrylic acid, or allylamine) which functionalize the surface of the silk materials with poly(acrylic acid) (PAA), poly(methacrylic acid) (PMAA), or poly(allylamine) (PAAm). To demonstrate potential applications of this type of modification, the polymer-modified silks are mineralized. The PAA- and PMAA-functionalized silks are mineralized with calcium carbonate, whereas the PAAm-functionalized silks are mineralized with silica, both of which provide a coating on the materials that may be useful for bone tissue engineering, which will be the subject of future investigations.
KW  - biomaterials
KW  - chemical modification
KW  - photochemistry
KW  - silkworm silk
KW  - spider silk
Y1  - 2018
U6  - https://doi.org/10.1002/mabi.201800216
SN  - 1616-5187
SN  - 1616-5195
VL  - 18
IS  - 11
PB  - Wiley-VCH
CY  - Weinheim
ER  -