TY  - JOUR
A1  - Rosencrantz, Ruben R.
A1  - Vu Hoa Nguyen, 
A1  - Park, Hyunji
A1  - Schulte, Christine
A1  - Böker, Alexander
A1  - Schnakenberg, Uwe
A1  - Elling, Lothar
T1  - Lectin binding studies on a glycopolymer brush flow-through biosensor by localized surface plasmon resonance
JF  - Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis
N2  - A localized surface plasmon resonance biosensor in a flow-through configuration was applied for investigating kinetics of lectin binding to surface-grafted glycopolymer brushes. Polycarbonate filter membranes with pore sizes of 400 nm were coated with a 114-nm thick gold layer and used as substrate for surface-initiated atom-transfer radical polymerization of a glycomonomer. These grafted from glycopolymer brushes were further modified with two subsequent enzymatic reactions on the surface to yield an immobilized trisaccharide presenting brush. Specific binding of lectins including Clostridium difficile toxin A receptor domain to the glycopolymer brush surface could be investigated in a microfluidic setup with flow-through of the analytes and transmission surface plasmon resonance spectroscopy.
KW  - Localized surface plasmon resonance
KW  - Glycopolymer brush
KW  - Microfluidics
KW  - Bacterial toxin
KW  - Glycosyltransferase
KW  - Biosensors
Y1  - 2016
U6  - https://doi.org/10.1007/s00216-016-9667-9
SN  - 1618-2642
SN  - 1618-2650
VL  - 408
SP  - 5633
EP  - 5640
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Hasinovic, H.
A1  - Friberg, S. E.
A1  - Kovach, Ildyko
A1  - Koetz, Joachim
T1  - Destabilization of a dual emulsion to form a Janus emulsion
JF  - Colloid and polymer science : official journal of the Kolloid-Gesellschaft
N2  - A vegetable oil (VO) was added to an emulsion of silicone oil in water (SO/W) with mixing limited to once turning the test tube upside down. Initially, the VO was dispersed into virtually centimeter-sized drops and the emulsion contained effectively no Janus drops, while after 1 h of agitation at a low level to prevent creaming, drops of 50-100-mu m size of the two oils were observed: in addition to an insignificant number of Janus drops. The topology of the latter showed them to emanate from flocculated individual drops of the two oils, but with no discernible effect by the interfacial tension equilibrium on the drop topology. Continued gentle mixing gave increasing fraction of Janus drops of increased size with a topology gradually approaching the one expected from the interfacial equilibrium at the contact line. The spontaneous formation of Janus drops indicated a reduction of the interfacial free energy in the process and the interfacial energy difference between separate and Janus drops was calculated for an appropriate range of interfacial tensions and for all oil fractions. The calculations enabled a distinction of the decrease due to interfacial area changes from the reduction of interfacial tensions per se, with the latter only a minor fraction.
KW  - Emulsion destabilization
KW  - Janus emulsions
KW  - Interfacial equilibrium
KW  - Microfluidics
Y1  - 2014
U6  - https://doi.org/10.1007/s00396-014-3263-3
SN  - 0303-402X
SN  - 1435-1536
VL  - 292
IS  - 9
SP  - 2319
EP  - 2324
PB  - Springer
CY  - New York
ER  - 
TY  - JOUR
A1  - Beta, Carsten
A1  - Bodenschatz, Eberhard
T1  - Microfluidic tools for quantitative studies of eukaryotic chemotaxis
JF  - European journal of cell biology
N2  - Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control. However, the times for built-up and switching of gradient profiles are in general too slow to resolve the intracellular protein translocation events of directional sensing of eukaryotes. Here, we review an example of a conventional microfluidic gradient mixer as well as the novel flow photolysis technique that achieves an increased temporal resolution by combining the photo-activation of caged compounds with the advantages of microfluidic chambers.
KW  - Eukaryotic chemotaxis
KW  - Dictyostelium discoideum
KW  - Microfluidics
KW  - Caged compounds
KW  - Numerical simulations
Y1  - 2011
U6  - https://doi.org/10.1016/j.ejcb.2011.05.006
SN  - 0171-9335
VL  - 90
IS  - 10
SP  - 811
EP  - 816
PB  - Elsevier
CY  - Jena
ER  -