TY  - JOUR
A1  - Dey, Pradip
A1  - Adamovski, Miriam
A1  - Friebe, Simon
A1  - Badalyan, Artavazd
A1  - Mutihac, Radu-Cristian
A1  - Paulus, Florian
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
A1  - Haag, Rainer
T1  - Dendritic polyglycerol-poly(ethylene glycol)-based polymer networks for biosensing application
JF  - ACS applied materials & interfaces
N2  - This work describes the formation of a new dendritic polyglycerol-poly(ethylene glycol)-based 3D polymer network as a matrix for immobilization of the redox enzyme periplasmatic aldehyde oxidoreductase to create an electrochemical biosensor. The novel network is built directly on the gold surface, where it simultaneously stabilizes the enzyme for up to 4 days. The prepared biosensors can be used for amperometric detection of benzaldehyde in the range of 0.8-400 mu M.
KW  - biosensors
KW  - hydrogel
KW  - amperometry
KW  - dendritic
Y1  - 2014
U6  - https://doi.org/10.1021/am502018x
SN  - 1944-8244
VL  - 6
IS  - 12
SP  - 8937
EP  - 8941
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Höfs, Soraya
A1  - Huelague, Deniz
A1  - Bennet, Francesca
A1  - Carl, Peter
A1  - Flemig, Sabine
A1  - Schmid, Thomas
A1  - Schenk, Jorg A.
A1  - Hodoroaba, Vasile-Dan
A1  - Schneider, Rudolf J.
T1  - Electrochemical immunomagnetic Ochratoxin A sensing
BT  - steps forward in the application of 3,3’,5,5’-Tetramethylbenzidine in amperometric assays
JF  - ChemElectroChem
N2  - Electrochemical methods offer great promise in meeting the demand for user-friendly on-site devices for monitoring important parameters. The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. Enzyme-linked immunosorbent assays, with photometric detection of 3,3',5,5'-tetramethylbenzidine (TMB), form a good basis for sensitive detection. To provide a straightforward approach for the miniaturization of the detection step, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. We created a smartphone-based, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis for sensing of further analytes.
KW  - amperometry
KW  - cyclic voltammetry
KW  - immunoassays
KW  - screen-printed electrodes
KW  - 3,3′,5,5′-tetramethylbenzidine
Y1  - 2021
U6  - https://doi.org/10.1002/celc.202100446
SN  - 2196-0216
VL  - 8
IS  - 13
SP  - 2597
EP  - 2606
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Othman, Abdelmageed M.
A1  - Wollenberger, Ulla
T1  - Amperometric biosensor based on coupling aminated laccase to functionalized carbon nanotubes for phenolics detection
JF  - International journal of biological macromolecules
N2  - A biosensor for phenolic compounds based on a chemically modified laccase from Coriolus hirsula immobilized on functionalized screen-printed carbon electrodes (SPCEs) was achieved. Different enzyme modifications and immobilization strategies were analyzed. The electrochemical response of the immobilized laccase on SPCEs modified with carboxyl functionalized multi-walled carbon nanotubes (COOH-MWCNT) was the highest when laccase was aminated prior to the adsorption onto the working electrode. The developed lactase biosensor sensitivity toward different phenolic compounds was assessed to determine the biosensor response with several phenolic compounds. The highest response was obtained for ABTS with a saturation value of I-max = 27.94 mu A. The electrocatalytic efficiency (I-max/K-m(app)) was the highest for ABTS (5588 mu A mu M-1) followed by syringaldazine (3014 mu A.mu M-1). The sensors were considerably stable, whereby 99.5, 82 and 77% of the catalytic response using catechol as substrate was retained after 4, 8 and 10 successive cycles of reuse respectively, with response time average of 5 s for 12 cycles. No loss of activity was observed after 20 days of storage.
KW  - amperometry
KW  - laccase
KW  - amination
KW  - biosensor
KW  - carbon nanotubes
KW  - phenols
Y1  - 2020
U6  - https://doi.org/10.1016/j.ijbiomac.2020.03.049
SN  - 0141-8130
SN  - 1879-0003
VL  - 153
SP  - 855
EP  - 864
PB  - Elsevier
CY  - New York, NY [u.a.]
ER  -