TY - GEN A1 - Hanack, Katja A1 - Schloer, Anja A1 - Holzloehner, Pamela A1 - Listek, Martin A1 - Bauer, Cindy A1 - Butze, Monique A1 - Micheel, Burkhard A1 - Hentschel, Christian A1 - Sowa, Mandy A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Fuener, Jonas A1 - Schliebs, Erik A1 - Goihl, Alexander A1 - Reinhold, Dirk T1 - Camelid nanobodies specific to human pancreatic glycoprotein 2 T2 - The journal of immunology N2 - Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified to be a major autoantigenic target in Crohn’s disease patients. It was discussed recently that a long and a short isoform of GP2 exists whereas the short isoform is often detected by GP2-specific autoantibodies. In the outcome of inflammatory bowel diseases, these GP2-specific autoantibodies are discussed as new serological markers for diagnosis and therapeutic monitoring. To investigate this further, camelid nanobodies were generated by phage display and selected against the short isoform of GP2 in order to isolate specific tools for the discrimination of both isoforms. Nanobodies are single domain antibodies derived from camelid heavy chain only antibodies and characterized by a high stability and solubility. The selected candidates were expressed, purified and validated regarding their binding properties in different enzyme-linked immunosorbent assays formats, immunofluorescence, immunohistochemistry and surface plasmon resonance spectroscopy. Four different nanobodies could be selected whereof three recognize the short isoform of GP2 very specifically and one nanobody showed a high binding capacity for both isoforms. The KD values measured for all nanobodies were between 1.3 nM and 2.3 pM indicating highly specific binders suitable for the application as diagnostic tool in inflammatory bowel disease. Y1 - 2016 SN - 0022-1767 SN - 1550-6606 VL - 196 SP - 313 EP - 328 PB - American Assoc. of Immunologists CY - Bethesda ER - TY - CHAP A1 - Kurbel, Karl A1 - Nowak, Dawid A1 - Azodi, Amir A1 - Jaeger, David A1 - Meinel, Christoph A1 - Cheng, Feng A1 - Sapegin, Andrey A1 - Gawron, Marian A1 - Morelli, Frank A1 - Stahl, Lukas A1 - Kerl, Stefan A1 - Janz, Mariska A1 - Hadaya, Abdulmasih A1 - Ivanov, Ivaylo A1 - Wiese, Lena A1 - Neves, Mariana A1 - Schapranow, Matthieu-Patrick A1 - Fähnrich, Cindy A1 - Feinbube, Frank A1 - Eberhardt, Felix A1 - Hagen, Wieland A1 - Plauth, Max A1 - Herscheid, Lena A1 - Polze, Andreas A1 - Barkowsky, Matthias A1 - Dinger, Henriette A1 - Faber, Lukas A1 - Montenegro, Felix A1 - Czachórski, Tadeusz A1 - Nycz, Monika A1 - Nycz, Tomasz A1 - Baader, Galina A1 - Besner, Veronika A1 - Hecht, Sonja A1 - Schermann, Michael A1 - Krcmar, Helmut A1 - Wiradarma, Timur Pratama A1 - Hentschel, Christian A1 - Sack, Harald A1 - Abramowicz, Witold A1 - Sokolowska, Wioletta A1 - Hossa, Tymoteusz A1 - Opalka, Jakub A1 - Fabisz, Karol A1 - Kubaczyk, Mateusz A1 - Cmil, Milena A1 - Meng, Tianhui A1 - Dadashnia, Sharam A1 - Niesen, Tim A1 - Fettke, Peter A1 - Loos, Peter A1 - Perscheid, Cindy A1 - Schwarz, Christian A1 - Schmidt, Christopher A1 - Scholz, Matthias A1 - Bock, Nikolai A1 - Piller, Gunther A1 - Böhm, Klaus A1 - Norkus, Oliver A1 - Clark, Brian A1 - Friedrich, Björn A1 - Izadpanah, Babak A1 - Merkel, Florian A1 - Schweer, Ilias A1 - Zimak, Alexander A1 - Sauer, Jürgen A1 - Fabian, Benjamin A1 - Tilch, Georg A1 - Müller, David A1 - Plöger, Sabrina A1 - Friedrich, Christoph M. A1 - Engels, Christoph A1 - Amirkhanyan, Aragats A1 - van der Walt, Estée A1 - Eloff, J. H. P. A1 - Scheuermann, Bernd A1 - Weinknecht, Elisa ED - Meinel, Christoph ED - Polze, Andreas ED - Oswald, Gerhard ED - Strotmann, Rolf ED - Seibold, Ulrich ED - Schulzki, Bernhard T1 - HPI Future SOC Lab BT - Proceedings 2015 N2 - Das Future SOC Lab am HPI ist eine Kooperation des Hasso-Plattner-Instituts mit verschiedenen Industriepartnern. Seine Aufgabe ist die Ermöglichung und Förderung des Austausches zwischen Forschungsgemeinschaft und Industrie. Am Lab wird interessierten Wissenschaftlern eine Infrastruktur von neuester Hard- und Software kostenfrei für Forschungszwecke zur Verfügung gestellt. Dazu zählen teilweise noch nicht am Markt verfügbare Technologien, die im normalen Hochschulbereich in der Regel nicht zu finanzieren wären, bspw. Server mit bis zu 64 Cores und 2 TB Hauptspeicher. Diese Angebote richten sich insbesondere an Wissenschaftler in den Gebieten Informatik und Wirtschaftsinformatik. Einige der Schwerpunkte sind Cloud Computing, Parallelisierung und In-Memory Technologien. In diesem Technischen Bericht werden die Ergebnisse der Forschungsprojekte des Jahres 2015 vorgestellt. Ausgewählte Projekte stellten ihre Ergebnisse am 15. April 2015 und 4. November 2015 im Rahmen der Future SOC Lab Tag Veranstaltungen vor. KW - Future SOC Lab KW - Forschungsprojekte KW - Multicore Architekturen KW - In-Memory Technologie KW - Cloud Computing KW - maschinelles Lernen KW - künstliche Intelligenz Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-102516 ER - TY - GEN A1 - Low, Thomas A1 - Hentschel, Christian A1 - Stober, Sebastian A1 - Sack, Harald A1 - Nürnberger, Andreas ED - Amsaleg, Laurent ED - Guðmundsson, Gylfi Þór ED - Gurrin, Cathal ED - Jónsson, Björn Þór ED - Satoh, Shin'ichi T1 - Exploring large movie collections BT - comparing visual berrypicking and traditional browsing T2 - Lecture notes in computer science N2 - We compare Visual Berrypicking, an interactive approach allowing users to explore large and highly faceted information spaces using similarity-based two-dimensional maps, with traditional browsing techniques. For large datasets, current projection methods used to generate maplike overviews suffer from increased computational costs and a loss of accuracy resulting in inconsistent visualizations. We propose to interactively align inexpensive small maps, showing local neighborhoods only, which ideally creates the impression of panning a large map. For evaluation, we designed a web-based prototype for movie exploration and compared it to the web interface of The Movie Database (TMDb) in an online user study. Results suggest that users are able to effectively explore large movie collections by hopping from one neighborhood to the next. Additionally, due to the projection of movie similarities, interesting links between movies can be found more easily, and thus, compared to browsing serendipitous discoveries are more likely. KW - Exploratory interfaces KW - Media retrieval KW - Multidimensional scaling KW - User study Y1 - 2016 SN - 978-3-319-51814-5 SN - 978-3-319-51813-8 U6 - https://doi.org/10.1007/978-3-319-51814-5_17 SN - 0302-9743 SN - 1611-3349 VL - 10133 SP - 198 EP - 208 PB - Springer CY - Cham ER - TY - JOUR A1 - Roggenbuck, Dirk A1 - Goihl, Alexander A1 - Hanack, Katja A1 - Holzloehner, Pamela A1 - Hentschel, Christian A1 - Veiczi, Miklos A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Schulz, Hans-Ulrich T1 - Serological diagnosis and prognosis of severe acute pancreatitis by analysis of serum glycoprotein 2 JF - Clinical chemistry and laboratory medicine : journal of the Forum of the European Societies of Clinical Chemistry - the European Branch of the International Federation of Clinical Chemistry and Laboratory Medicine N2 - To better understand emerging adults’ perceptions of family interactions and value transmission to the next generation, we examined Hmong American emerging adults’ reflections on their parents’ parenting. Participants discussed what parenting practices they would do differently and others they hoped to emulate with their future adolescent children. Thirty Hmong American emerging adults (18-25 years; M = 21.2 years; 50% female) participated in interviews that focused retrospectively on the parent–adolescent relationship. Results revealed that emerging adults wanted to parent differently in three ways: less pressure about education, fewer restrictions, and more open communication. Emerging adults imagined being a similar parent in four ways: promoting education, promoting life values, giving guidance, and offering love and support. The findings highlight parenting practices that Hmong American emerging adults plan on transmitting (and not transmitting) to their own children, offering a glimpse into the type of parents the emerging adults may become. KW - acute pancreatitis KW - chronic pancreatitis KW - GP2 isoform alpha KW - pancreatic neoplasms KW - severe acute pancreatitis KW - zymogen granule membrane glycoprotein GP2 Y1 - 2017 U6 - https://doi.org/10.1515/cclm-2016-0797 SN - 1434-6621 SN - 1437-4331 VL - 55 SP - 854 EP - 864 PB - De Gruyter CY - Berlin ER - TY - JOUR A1 - Schlör, Anja A1 - Holzlöhner, Pamela A1 - Listek, Martin A1 - Grieß, Cindy A1 - Butze, Monique A1 - Micheel, Burkhard A1 - Hentschel, Christian A1 - Sowa, Mandy A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Füner, Jonas A1 - Schliebs, Erik A1 - Goihl, Alexander A1 - Reinhold, Dirk A1 - Hanack, Katja T1 - Generation and validation of murine monoclonal and camelid recombinant single domain antibodies specific for human pancreatic glycoprotein 2 JF - New biotechnology N2 - Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified as a major autoantigenic target in Crohn’s disease patients. It was reported recently that a long (GP2a) and a short (GP2b) isoform of GP2 exist and that in the outcome of inflammatory bowel diseases (IBD) GP2-specific autoantibodies probably appear as new serological markers for diagnosis and therapeutic monitoring. To investigate this further and in order to establish diagnostic tools for the discrimination of both GP2 isoforms, a set of different murine monoclonal and camelid recombinant single domain antibodies (camelid VHH) was generated and validated in various enzyme-linked immunosorbent assay (ELISA) formats, immunofluorescence on transgenic cell lines and immunohistochemistry on monkey pancreas tissue sections. Out of six binders identified, one was validated as highly specific for GP2a. This murine monoclonal antibody (mAb) was used as capture antibody in construction of a sandwich ELISA for the detection of GP2a. Camelid VHHs or a second murine mAb served as detection antibodies in this system. All antibodies were also able to stain GP2a or GP2b on transgenic cell lines as well as on pancreatic tissue in immunohistochemistry. The KD values measured for the camelid VHHs were between 7 nM and 23pM. This set of specific binders will enable the development of suitable diagnostic tools for GP2-related studies in IBD. KW - glycoprotein GP2 KW - Monoclonal antibodies KW - Camelid single domain antibodies Y1 - 2018 U6 - https://doi.org/10.1016/j.nbt.2018.03.006 SN - 1871-6784 SN - 1876-4347 VL - 45 SP - 60 EP - 68 PB - Elsevier CY - Amsterdam ER -