TY  - GEN
A1  - Westendorf, Christian
A1  - Bae, Albert J.
A1  - Erlenkamper, Christoph
A1  - Galland, Edouard
A1  - Franck, Carl
A1  - Bodenschatz, Eberhard
A1  - Beta, Carsten
T1  - Live cell flattening
BT  - traditional and novel approaches
T2  - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2  - Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 835 
KW  - PDMS
KW  - microfluidic device
KW  - lower channel
KW  - total internal reflection fluorescence
KW  - total internal reflection fluorescence microscopy
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-428311
SN  - 1866-8372
IS  - 835
ER  - 
TY  - GEN
A1  - Beta, Carsten
T1  - Bistability in the actin cortex
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - Multi-color fluorescence imaging experiments of wave forming Dictyostelium cells have revealed that actin waves separate two domains of the cell cortex that differ in their actin structure and phosphoinositide composition. We propose a bistable model of actin dynamics to account for these experimental observation. The model is based on the simplifying assumption that the actin cytoskeleton is composed of two distinct network types, a dendritic and a bundled network. The two structurally different states that were observed in experiments correspond to the stable fixed points in the bistable regime of this model. Each fixed point is dominated by one of the two network types. The experimentally observed actin waves can be considered as trigger waves that propagate transitions between the two stable fixed points.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 756 
KW  - stable fixed point
KW  - dendritic network
KW  - total internal reflection fluorescence microscopy
KW  - dictyostelium cell
KW  - bistable regime
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-429385
VL  - 3
IS  - 12
ER  -