TY  - JOUR
A1  - Yarman, Aysu
T1  - Electrosynthesized Molecularly Imprinted Polymer for Laccase Using the Inactivated Enzyme as the Target
JF  - Bulletin of the Korean chemical society
N2  - The first molecularly imprinted polymer (MIP) for the recognition of the copper-enzyme laccase was successfully prepared by electropolymerizing scopoletin in the presence of alkaline-inactivated enzyme. Laccase-MIP and the control polymer without laccase (nonimprinted polymer, NIP) were characterized by voltammetry using the redox marker ferricyanide. After electropolymerization, the signals for ferricyanide for both the MIP and the NIP were almost completely suppressed and increased after removal of the target from the polymer layer. Rebinding of both inactivated and active laccase decreased the ferricyanide peak currents to almost equal extent. The relative decrease of signal suppression approached saturation above 10 nM. Furthermore, the surface activity of rebound laccase toward the oxidation of catechol was investigated. The surface activity approached saturation above 10 nM, a value close to the value of the measurements with ferricyanide. Interaction of NIP with laccase brought about a six times smaller signal of catechol oxidation.
KW  - Molecularly imprinted polymers
KW  - Biomimetic sensors
KW  - Laccase
KW  - Electropolymerization
KW  - Scopoletin
Y1  - 2018
U6  - https://doi.org/10.1002/bkcs.11413
SN  - 1229-5949
VL  - 39
IS  - 4
SP  - 483
EP  - 488
PB  - Wiley-VCH
CY  - Weinheim
ER  -