TY  - JOUR
A1  - Lämke, Jörn
A1  - Brzezinka, Krzysztof
A1  - Altmann, Simone
A1  - Bäurle, Isabel
T1  - A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory
JF  - The EMBO journal
N2  - In nature, plants often encounter chronic or recurring stressful conditions. Recent results indicate that plants can remember a past exposure to stress to be better prepared for a future stress incident. However, the molecular basis of this is poorly understood. Here, we report the involvement of chromatin modifications in the maintenance of acquired thermotolerance (heat stress [HS] memory). HS memory is associated with the accumulation of histone H3 lysine 4 di- and trimethylation at memory-related loci. This accumulation outlasts their transcriptional activity and marks them as recently transcriptionally active. High accumulation of H3K4 methylation is associated with hyper-induction of gene expression upon a recurring HS. This transcriptional memory and the sustained accumulation of H3K4 methylation depend on HSFA2, a transcription factor that is required for HS memory, but not initial heat responses. Interestingly, HSFA2 associates with memory-related loci transiently during the early stages following HS. In summary, we show that transcriptional memory after HS is associated with sustained H3K4 hyper-methylation and depends on a hit-and-run transcription factor, thus providing a molecular framework for HS memory.
KW  - chromatin
KW  - H3K4 methylation
KW  - heat shock transcription factor
KW  - priming
KW  - transcriptional memory
Y1  - 2016
U6  - https://doi.org/10.15252/embj.201592593
SN  - 0261-4189
SN  - 1460-2075
VL  - 35
SP  - 162
EP  - 175
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -