TY  - JOUR
A1  - Laux, Eva-Maria
A1  - Bier, Frank Fabian
A1  - Hölzel, Ralph
T1  - Dielectrophoretic Stretching of DNA
JF  - DNA Nanotechnology
N2  - The spatial control of DNA and of self-assembled DNA constructs is a prerequisite for the preparation of DNA-based nanostructures and microstructures and a useful tool for studies on single DNA molecules. Here we describe a protocol for the accumulation of dissolved lambda-DNA molecules between planar microelectrodes by the action of inhomogeneous radiofrequency electric fields. The resulting AC electrokinetic forces stretch the DNA molecules and align them parallel to the electric field. The electrode preparation from off-the-shelf electronic components is explained, and a detailed description of the electronic setup is given. The experimental procedure is controlled in real-time by fluorescence microscopy.
KW  - Alignment
KW  - Dielectrophoresis
KW  - DNA
KW  - Electrokinetics
KW  - Interdigitated electrodes
KW  - Stretching
Y1  - 2018
SN  - 978-1-4939-8582-1
SN  - 978-1-4939-8581-4
U6  - https://doi.org/10.1007/978-1-4939-8582-1_14
SN  - 1064-3745
SN  - 1940-6029
SP  - 199
EP  - 208
PB  - Humana Press Inc.
CY  - New York
ET  - 2
ER  - 
TY  - JOUR
A1  - Laux, Eva-Maria
A1  - Bier, Frank Fabian
A1  - Hölzel, Ralph
T1  - Electrode-based AC electrokinetics of proteins
BT  - a mini-review
JF  - Bioelectrochemistry : official journal of the Bioelectrochemical Society ; an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry
N2  - Employing electric phenomena for the spatial manipulation of bioparticles from whole cells down to dissolved molecules has become a useful tool in biotechnology and analytics. AC electrokinetic effects like dielectrophoresis and AC electroosmosis are increasingly used to concentrate, separate and immobilize DNA and proteins. With the advance of photolithographical micro- and nanofabrication methods, novel or improved bioanalytical applications benefit from concentrating analytes, signal enhancement and locally controlled immobilization by AC electrokinetic effects. In this review of AC electrokinetics of proteins, the respective studies are classified according to their different electrode geometries: individual electrode pairs, interdigitated electrodes, quadrupole electrodes, and 3D configurations of electrode arrays. Known advantages and disadvantages of each layout are discussed.
KW  - AC electrokinetics
KW  - Dielectrophoresis
KW  - Electrodes
KW  - Electroosmosis
KW  - Proteins
Y1  - 2017
U6  - https://doi.org/10.1016/j.bioelechem.2017.11.010
SN  - 1567-5394
SN  - 1878-562X
VL  - 120
SP  - 76
EP  - 82
PB  - Elsevier B.V.
CY  - Amsterdam
ER  -