TY  - JOUR
A1  - Reinicke, Stefan
A1  - Rees, Huw C.
A1  - Espeel, Pieter
A1  - Vanparijs, Nane
A1  - Bisterfeld, Carolin
A1  - Dick, Markus
A1  - Rosencrantz, Ruben R.
A1  - Brezesinski, Gerald
A1  - de Geest, Bruno G.
A1  - Du Prez, Filip E.
A1  - Pietruszka, Jörg
A1  - Böker, Alexander
T1  - Immobilization of 2-Deoxy-D-ribose-5-phosphate Aldolase in Polymeric Thin Films via the Langmuir-Schaefer Technique
JF  - ACS applied materials & interfaces
N2  - A synthetic protocol for the fabrication of ultrathin polymeric films containing the enzyme 2-deoxy-D-ribose-5-phosphate aldolase from Escherichia coli (DERA(EC)) is presented. Ultrathin enzymatically active films are useful for applications in which only small quantities of active material are needed and at the same time quick response and contact times without diffusion limitation are wanted. We show how DERA as an exemplary enzyme can be immobilized in a thin polymer layer at the air-water interface and transferred to a suitable support by the Langmuir-Schaefer technique under full conservation of enzymatic activity. The polymer in use is a poly(N-isopropylacrylamide-co-N-2-thiolactone acrylamide) (P(NIPAAm-co-TlaAm)) statistical copolymer in which the thiolactone units serve a multitude of purposes including hydrophobization of the polymer, covalent binding of the enzyme and the support and finally cross-linking of the polymer matrix. The application of this type of polymer keeps the whole approach simple as additional cocomponents such as cross-linkers are avoided.
KW  - Langmuir-Schaefer
KW  - enzyme immobilization
KW  - 2-deoxy-D-ribose-5-phosphate aldolase
KW  - polymeric thin film
KW  - poly(N-isopropylacrylamide)
KW  - thiolactone
Y1  - 2017
U6  - https://doi.org/10.1021/acsami.6b13632
SN  - 1944-8244
VL  - 9
SP  - 8317
EP  - 8326
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Zhang, Shuhao
A1  - Bisterfeld, Carolin
A1  - Bramski, Julia
A1  - Vanparijs, Nane
A1  - De Geest, Bruno G.
A1  - Pietruszka, Jörg
A1  - Böker, Alexander
A1  - Reinicke, Stefan
T1  - Biocatalytically Active Thin Films via Self-Assembly of 2-Deoxy-D-ribose-5-phosphate Aldolase-Poly(N-isopropylacrylamide) Conjugates
JF  - Bioconjugate chemistry
N2  - 2-Deoxy-D-ribose-5-phosphate aldolase (DERA) is a biocatalyst that is capable of converting acetaldehyde and a second aldehyde as acceptor into enantiomerically pure mono- and diyhydroxyaldehydes, which are important structural motifs in a number of pharmaceutically active compounds. However, substrate as well as product inhibition requires a more-sophisticated process design for the synthesis of these motifs. One way to do so is to the couple aldehyde conversion with transport processes, which, in turn, would require an immobilization of the enzyme within a thin film that can be deposited on a membrane support. Consequently, we developed a fabrication process for such films that is based on the formation of DERA-poly(N-isopropylacrylamide) conjugates that are subsequently allowed to self-assemble at an air-water interface to yield the respective film. In this contribution, we discuss the conjugation conditions, investigate the interfacial properties of the conjugates, and, finally, demonstrate a successful film formation under the preservation of enzymatic activity.
Y1  - 2017
U6  - https://doi.org/10.1021/acs.bioconjchem.7b00645
SN  - 1043-1802
VL  - 29
IS  - 1
SP  - 104
EP  - 116
PB  - American Chemical Society
CY  - Washington
ER  -