TY  - JOUR
A1  - Bauer, Christian G.
A1  - Eremenko, A. V.
A1  - Ehrentreich-Förster, Eva
A1  - Bier, Frank Fabian
A1  - Makower, Alexander
A1  - Halsall, H. B.
A1  - Heineman, W. R.
A1  - Scheller, Frieder W.
T1  - Zeptomole-detecting biosensor for alkaline phosphatase in an electroche mical immunoassay for 2,4- dichlorophenoacetic acid
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Schubert, Florian
A1  - Bier, Frank Fabian
T1  - Vom Biosensor zur Nanobiotechnologie
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Jetzschmann, Katharina J.
A1  - Jagerszki, Gyula
A1  - Dechtrirat, Decha
A1  - Yarman, Aysu
A1  - Gajovic-Eichelmann, Nenad
A1  - Gilsing, Hans-Detlev
A1  - Schulz, Burkhard
A1  - Gyurcsanyi, Robert E.
A1  - Scheller, Frieder W.
T1  - Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition
JF  - Advanced functional materials
N2  - Effective recognition of enzymatically active tetrameric acetylcholinesterase (AChE) is accomplished by a hybrid nanofilm composed of a propidium-terminated self-assembled monolayer (Prop-SAM) which binds AChE via its peripheral anionic site (PAS) and an ultrathin electrosynthesized molecularly imprinted polymer (MIP) cover layer of a novel carboxylate-modified derivative of 3,4-propylenedioxythiophene. The rebinding of the AChE to the MIP/Prop-SAM nanofilm covered electrode is detected by measuring in situ the enzymatic activity. The oxidative current of the released thiocholine is dependent on the AChE concentration from approximate to 0.04 x 10(-6) to 0.4 x 10(-6)m. An imprinting factor of 9.9 is obtained for the hybrid MIP, which is among the best values reported for protein imprinting. The dissociation constant characterizing the strength of the MIP-AChE binding is 4.2 x 10(-7)m indicating the dominant role of the PAS-Prop-SAM interaction, while the benefit of the MIP nanofilm covering the Prop-SAM layer is the effective suppression of the cross-reactivity toward competing proteins as compared with the Prop-SAM. The threefold selectivity gain provided by i) the shape-specific MIP filter, ii) the propidium-SAM, iii) signal generation only by the AChE bound to the nanofilm shows promise for assessing AChE activity levels in cerebrospinal fluid.
KW  - acetylcholinesterase
KW  - biomimetic sensors
KW  - molecularly imprinted electropolymers
KW  - peripheral anionic site
KW  - propidium
Y1  - 2015
U6  - https://doi.org/10.1002/adfm.201501900
SN  - 1616-301X
SN  - 1616-3028
VL  - 25
IS  - 32
SP  - 5178
EP  - 5183
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Asche, Hartmut
A1  - Böckmann, Christine
A1  - Laue, Steffen
A1  - Löhmannsröben, Hans-Gerd
A1  - Lemke, Matthias
A1  - Schober, Lars
A1  - Reich, Oliver
A1  - Lück, Erika
A1  - Schütte, Marc
A1  - Domsch, Horst
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
A1  - Stöcklein, Wolfgang
A1  - Wollenberger, Ursula
A1  - Schultze, Rainer
A1  - Hengstermann, Theo
A1  - Schael, Frank
T1  - Umweltforschung für das Land Brandenburg : Projekt Umweltanalytik / Umweltmeßtechnik / Informationssysteme
Y1  - 2000
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-3862
SP  - 176
EP  - 227
ER  - 
TY  - CHAP
A1  - Lück, Erika
A1  - Balderjahn, Ingo
A1  - Kamm, Birgit
A1  - Greil, Holle
A1  - Wallschläger, Hans-Dieter
A1  - Jessel, Beate
A1  - Böckmann, Christine
A1  - Oberhänsli, Roland
A1  - Soyez, Konrad
A1  - Schmeer, Ernst
A1  - Blumenstein, Oswald
A1  - Berndt, Klaus-Peter
A1  - Edeling, Thomas
A1  - Friedrich, Sabine
A1  - Kaden, Klaus
A1  - Scheller, Frieder W.
A1  - Petersen, Hans-Georg
A1  - Asche, Hartmut
A1  - Bronstert, Axel
A1  - Giest, Hartmut
A1  - Gaedke, Ursula
A1  - Löhmannsröben, Hans-Gerd
A1  - Jeltsch, Florian
A1  - Jänkel, Ralph
A1  - Gzik, Axel
A1  - Bork, Hans-Rudolf
A1  - Bork, Hans-Rudolf
T1  - Umweltforschung für das Land Brandenburg : Arbeitsgruppen und Professuren
Y1  - 2000
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-3797
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Eremenko, A. V.
A1  - Wollenberger, Ursula
A1  - Bauer, Christian G.
A1  - Pfeiffer, Dorothea
A1  - Micheel, Burkhard
T1  - Ultrasensitive biosensors
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Szeponik, Jan
A1  - Möller, B.
A1  - Pfeiffer, Dorothea
A1  - Lisdat, Fred
A1  - Wollenberger, Ursula
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Ultrasensitive bienzyme sensor for adrenaline
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Makower, Alexander
A1  - Eremenko, A. V.
A1  - Streffer, Katrin
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Tyrosinase-glucose dehydrogenase substrate-recycling biosensor : a highly sensitive measurement of phenolic compounds
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
T1  - Tribute to Guenter Gauglitz (Editorial)
Y1  - 2009
UR  - http://www.springerlink.com/content/100417
U6  - https://doi.org/10.1007/s00216-008-2548-0
SN  - 1618-2642
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Trends in der Bioanalytik
Y1  - 2002
ER  -