TY  - GEN
A1  - Scarpeci, Telma E.
A1  - Zanor, María I.
A1  - Carrillo, Néstor
A1  - Mueller-Roeber, Bernd
A1  - Valle, Estela M.
T1  - Generation of superoxide anion in chloroplasts of Arabidopsis thaliana during active photosynthesis
BT  - a focus on rapidly induced genes
T2  - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2  - The antioxidant defense system involves complex functional coordination of multiple components in different organelles within the plant cell. Here, we have studied the Arabidopsis thaliana early response to the generation of superoxide anion in chloroplasts during active photosynthesis. We exposed plants to methyl viologen (MV), a superoxide anion propagator in the light, and performed biochemical and expression profiling experiments using Affymetrix ATH1 GeneChip(R) microarrays under conditions in which photosynthesis and antioxidant enzymes were active. Data analysis identified superoxide-responsive genes that were compared with available microarray results. Examples include genes encoding proteins with unknown function, transcription factors and signal transduction components. A common GAAAAGTCAAAC motif containing the W-box consensus sequence of WRKY transcription factors, was found in the promoters of genes highly up-regulated by superoxide. Band shift assays showed that oxidative treatments enhanced the specific binding of leaf protein extracts to this motif. In addition, GUS reporter gene fused to WRKY30 promoter, which contains this binding motif, was induced by MV and H2O2. Overall, our study suggests that genes involved in signalling pathways and with unknown functions are rapidly activated by superoxide anion generated in photosynthetically active chloroplasts, as part of the early antioxidant response of Arabidopsis leaves.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 866 
KW  - antioxidant response
KW  - chloroplast
KW  - Hsp
KW  - oxidative stress
KW  - WRKY
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-434254
SN  - 1866-8372
IS  - 866
SP  - 361
EP  - 378
ER  - 
TY  - BOOK
ED  - Kosta, Peter
ED  - Schürcks, Lilia
T1  - Linguistic Investigations into Formal Description of Slavic Languages
BT  - Contributions of the sixth European Conference held at Potsdam University, November 30 - December 02, 2005
T3  - Potsdam Linguistic Investigations
Y1  - 2007
SN  - 978-3-631-55376-3
VL  - 1
PB  - Peter Lang
CY  - Frankfurt am Main
ER  - 
TY  - BOOK
ED  - Kliegl, Reinhold
T1  - Conference Abstracts : ECEM 2007 ; 14. European Conference on Eye Movements ECEM2007, Potsdam, August 19 - 23, 2007
T3  - Journal of eye movement research : Special issue
Y1  - 2007
UR  - http://www.jemr.org/online/1/s1
SN  - 1995-8692
VL  - 1
PB  - Universität
CY  - Potsdam
ER  - 
TY  - JOUR
A1  - Engbert, Ralf
A1  - Nuthmann, Antje
A1  - Kliegl, Reinhold
T1  - An lterative algorithm for the estimation of the distribution of mislocated fixations during reading
Y1  - 2007
SN  - 978-0-08-044980-7
ER  - 
TY  - JOUR
A1  - Bohn, Christiane
A1  - Kliegl, Reinhold
T1  - Post-interpretive processes influence interpretive processing during reading: evidence from eye movements
Y1  - 2007
SN  - 978-18-4169-96-9
ER  - 
TY  - JOUR
A1  - Schroeder, Christoph
T1  - Boeschoten, H., Johanson, L. (Hrsg.), Turkic languages in contact; Wiesbaden, Harrassowitz, 2006
BT  - Turkic languages in contact
Y1  - 2007
ER  - 
TY  - JOUR
A1  - Wiemann, Dirk
T1  - From Forked Tongue to Forked Tongue : Rushdie and Milton in the Postcolonial conversation
Y1  - 2007
UR  - http://jcl.sagepub.com/content/42/2/47.full.pdf+html
SN  - 0021-9894
ER  - 
TY  - JOUR
A1  - Ette, Ottmar
T1  - Towards World Science? Humboldtian Science, World Concepts, and Transarea Studies
Y1  - 2007
SN  - 978-84-8489-303-5
ER  - 
TY  - JOUR
A1  - Schwarz, Anja
T1  - Strategic uses of multiculturalism in Germay and Australia
Y1  - 2007
SN  - 978-90-420-2307-9
ER  - 
TY  - JOUR
A1  - Lloyd, Justine
A1  - Schwarz, Anja
T1  - The Pacific Solution meets Fortress Europe : Emerging Parallels in Transnational Refugee Regimes
Y1  - 2007
SN  - 978-90-420-2307-9
ER  -