TY  - JOUR
A1  - de Abreu e Lima, Francisco Anastacio
A1  - Li, Kun
A1  - Wen, Weiwei
A1  - Yan, Jianbing
A1  - Nikoloski, Zoran
A1  - Willmitzer, Lothar
A1  - Brotman, Yariv
T1  - Unraveling lipid metabolism in maize with time-resolved multi-omics data
JF  - The plant journal
N2  - Maize is the cereal crop with the highest production worldwide, and its oil is a key energy resource. Improving the quantity and quality of maize oil requires a better understanding of lipid metabolism. To predict the function of maize genes involved in lipid biosynthesis, we assembled transcriptomic and lipidomic data sets from leaves of B73 and the high-oil line By804 in two distinct time-series experiments. The integrative analysis based on high-dimensional regularized regression yielded lipid-transcript associations indirectly validated by Gene Ontology and promoter motif enrichment analyses. The co-localization of lipid-transcript associations using the genetic mapping of lipid traits in leaves and seedlings of a B73 x By804 recombinant inbred line population uncovered 323 genes involved in the metabolism of phospholipids, galactolipids, sulfolipids and glycerolipids. The resulting association network further supported the involvement of 50 gene candidates in modulating levels of representatives from multiple acyl-lipid classes. Therefore, the proposed approach provides high-confidence candidates for experimental testing in maize and model plant species.
KW  - Zea mays
KW  - lipid metabolism
KW  - omics
KW  - GFLASSO
KW  - QTL
Y1  - 2018
U6  - https://doi.org/10.1111/tpj.13833
SN  - 0960-7412
SN  - 1365-313X
VL  - 93
IS  - 6
SP  - 1102
EP  - 1115
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Wu, Hao
A1  - Han, Yijie
A1  - Rodriguez Sillke, Yasmina
A1  - Deng, Hongzhang
A1  - Siddiqui, Sophiya
A1  - Treese, Christoph
A1  - Schmidt, Franziska
A1  - Friedrich, Marie
A1  - Keye, Jacqueline
A1  - Wan, Jiajia
A1  - Qin, Yue
A1  - Kühl, Anja A.
A1  - Qin, Zhihai
A1  - Siegmund, Britta
A1  - Glauben, Rainer
T1  - Lipid droplet-dependent fatty acid metabolism controls the immune suppressive phenotype of tumor-associated macrophages
JF  - EMBO molecular medicine
N2  - Tumor-associated macrophages (TAMs) promote tumor growth and metastasis by suppressing tumor immune surveillance. Herein, we provide evidence that the immunosuppressive phenotype of TAMs is controlled by long-chain fatty acid metabolism, specifically unsaturated fatty acids, here exemplified by oleate. Consequently, en-route enriched lipid droplets were identified as essential organelles, which represent effective targets for chemical inhibitors to block in vitro polarization of TAMs and tumor growth in vivo. In line, analysis of human tumors revealed that myeloid cells infiltrating colon cancer but not gastric cancer tissue indeed accumulate lipid droplets. Mechanistically, our data indicate that oleate-induced polarization of myeloid cells depends on the mammalian target of the rapamycin pathway. Thus, our findings reveal an alternative therapeutic strategy by targeting the pro-tumoral myeloid cells on a metabolic level.
KW  - cancer immunotherapy
KW  - lipid droplets
KW  - lipid metabolism
KW  - tumor microenvironment
KW  - tumor-associated macrophage
Y1  - 2019
U6  - https://doi.org/10.15252/emmm.201910698
SN  - 1757-4676
SN  - 1757-4684
VL  - 11
IS  - 11
PB  - Wiley
CY  - Hoboken
ER  -