TY  - GEN
A1  - Szarzynska, Bogna
A1  - Sobkowiak, Lukasz
A1  - Pant, Bikram Datt
A1  - Balazadeh, Salma
A1  - Scheible, Wolf-Rüdiger
A1  - Müller-Röber, Bernd
A1  - Jarmolowski, Artur
A1  - Szweykowska-Kulinska, Zofia
T1  - Gene structures and processing of Arabidopsis thaliana HYL1-dependent pri-miRNAs
N2  - Arabidopsis thaliana HYL1 is a nuclear doublestranded RNA-binding protein involved in the maturation of pri-miRNAs. A quantitative real-time PCR platform for parallel quantification of 176 primiRNAs was used to reveal strong accumulation of 57 miRNA precursors in the hyl1 mutant that completely lacks HYL1 protein. This approach enabled us for the first time to pinpoint particular members of MIRNA family genes that require HYL1 activity for efficient maturation of their precursors. Moreover, the accumulation of miRNA precursors in the hyl1 mutant gave us the opportunity to carry out 3’ and 5’ RACE experiments which revealed that some of these precursors are of unexpected length. The alignment of HYL1- dependent miRNA precursors to A. thaliana genomic sequences indicated the presence of introns in 12 out of 20 genes studied. Some of the characterized intron-containing pri-miRNAs undergo alternative splicing such as exon skipping or usage of alternative 5’ splice sites suggesting that this process plays a role in the regulation of miRNA biogenesis. In the hyl1 mutant intron-containing pri-miRNAs accumulate alongside spliced primiRNAs suggesting the recruitment of HYL1 into the miRNA precursor maturation pathway before their splicing occurs.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 138 
KW  - Binding-protein hyl1
KW  - Abscisic-acid
KW  - Flowering time
KW  - Micro-RNA
KW  - Serrate
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45085
ER  - 
TY  - JOUR
A1  - Olas, Justyna Jadwiga
A1  - Apelt, Federico
A1  - Watanabe, Mutsumi
A1  - Höfgen, Rainer
A1  - Wahl, Vanessa
T1  - Developmental stage-specific metabolite signatures in Arabidopsis thaliana under optimal and mild nitrogen limitation
JF  - Plant science : an international journal of experimental plant biology
N2  - Metabolites influence flowering time, and thus are among the major determinants of yield. Despite the reported role of trehalose 6-phosphate and nitrate signaling on the transition from the vegetative to the reproductive phase, little is known about other metabolites contributing and responding to developmental phase changes. To increase our understanding which metabolic traits change throughout development in Arabidopsis thaliana and to identify metabolic markers for the vegetative and reproductive phases, especially among individual amino acids (AA), we profiled metabolites of plants grown in optimal (ON) and limited nitrogen (N) (LN) conditions, the latter providing a mild but consistent limitation of N. We found that although LN plants adapt their growth to a decreased level of N, their metabolite profiles are strongly distinct from ON plant profiles, with N as the driving factor for the observed differences. We demonstrate that the vegetative and the reproductive phase are not only marked by growth parameters such as biomass and rosette area, but also by specific metabolite signatures including specific single AA. In summary, we identified N-dependent and -independent indicators manifesting developmental stages, indicating that the plant's metabolic status also reports on the developmental phases.
KW  - Amino acids
KW  - Floral induction
KW  - Flowering time
KW  - Nitrogen
KW  - Metabolites
KW  - Vegetative phase
KW  - Reproductive phase
Y1  - 2021
U6  - https://doi.org/10.1016/j.plantsci.2020.110746
SN  - 0168-9452
SN  - 1873-2259
VL  - 303
PB  - Elsevier Science
CY  - Amsterdam [u.a.]
ER  -