TY  - JOUR
A1  - Bartsch, Ingrid
A1  - Zschaler, Ingrid
A1  - Haseloff, Monika
A1  - Steinberg, Pablo
T1  - Establishment of a long-term culture system for rat colon epithelial cells
N2  - The aim of this study was to establish a long-term culture. system for rat colon epithelia isolaled by incubating a 4-cm-long rat colon segment cut longitudinally with all ethylenediaminetetraacetic acid [disodium salt]- containing buffer, taken up in conditioned medium from the normal rat kidney fibroblast cell line NRK (i.e., the supernatant Of pure NRK cultures), directly plated on mitomycin C-treated NRK cells and subcultured with conditioned medium from NRK cells. Cells started to migrate out of the crypts shortly after plating them on NRK feeder layers. Some of the crypts fell apart during the isolation procedure. whereas the vast majority of them did it within I to 2 Ill after plating. The cells proliferated extremely slowly but continuously over a period of 4 mo and were epithelial because they expressed cytokeratin 19 and were stained by crystal violet at pH 2.8. In conclusion, the experimental system described ill this study allows to maintain rat colon epithelial cells for up to 4 mo in culture and can be used to Study the effects of a variety of tumor-modulating factors on growth and gene expression of normal colon epithelial cells in vitro
Y1  - 2003
UR  - http://www.springerlink.com/content/120498/
U6  - https://doi.org/10.1290/0404035.1
SN  - 1071-2690
ER  - 
TY  - JOUR
A1  - Okano, J.
A1  - Shiota, G.
A1  - Matsumoto, K.
A1  - Yasui, S.
A1  - Kurimasa, A.
A1  - Hisatome, I.
A1  - Steinberg, Pablo
A1  - Murawaki, Y.
T1  - Hepatocyte growth factor exerts a proliferative effect on oval cells through the PI3K/AKT signaling pathway
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Stark, Avishay abraham
A1  - Porat, Noga
A1  - Volohonsky, Gloria
A1  - Konlosh, A.
A1  - Bluvshtein, Evgenia
A1  - Tubi, C.
A1  - Steinberg, Pablo
T1  - The role of gamma-glutamyl transpeptidase in the biosynthesis of glutathione
Y1  - 2003
ER  -