TY - GEN A1 - Beta, Carsten T1 - Bistability in the actin cortex T2 - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe N2 - Multi-color fluorescence imaging experiments of wave forming Dictyostelium cells have revealed that actin waves separate two domains of the cell cortex that differ in their actin structure and phosphoinositide composition. We propose a bistable model of actin dynamics to account for these experimental observation. The model is based on the simplifying assumption that the actin cytoskeleton is composed of two distinct network types, a dendritic and a bundled network. The two structurally different states that were observed in experiments correspond to the stable fixed points in the bistable regime of this model. Each fixed point is dominated by one of the two network types. The experimentally observed actin waves can be considered as trigger waves that propagate transitions between the two stable fixed points. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 756 KW - stable fixed point KW - dendritic network KW - total internal reflection fluorescence microscopy KW - dictyostelium cell KW - bistable regime Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-429385 VL - 3 IS - 12 ER - TY - GEN A1 - Breitenstein, Michael A1 - Hölzel, Ralph A1 - Bier, Frank Fabian T1 - Immobilization of different biomolecules by atomic force microscopy T2 - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe N2 - Background Micrometer resolution placement and immobilization of probe molecules is an important step in the preparation of biochips and a wide range of lab-on-chip systems. Most known methods for such a deposition of several different substances are costly and only suitable for a limited number of probes. In this article we present a flexible procedure for simultaneous spatially controlled immobilization of functional biomolecules by molecular ink lithography. Results For the bottom-up fabrication of surface bound nanostructures a universal method is presented that allows the immobilization of different types of biomolecules with micrometer resolution. A supporting surface is biotinylated and streptavidin molecules are deposited with an AFM (atomic force microscope) tip at distinct positions. Subsequent incubation with a biotinylated molecule species leads to binding only at these positions. After washing streptavidin is deposited a second time with the same AFM tip and then a second biotinylated molecule species is coupled by incubation. This procedure can be repeated several times. Here we show how to immobilize different types of biomolecules in an arbitrary arrangement whereas most common methods can deposit only one type of molecules. The presented method works on transparent as well as on opaque substrates. The spatial resolution is better than 400 nm and is limited only by the AFM's positional accuracy after repeated z-cycles since all steps are performed in situ without moving the supporting surface. The principle is demonstrated by hybridization to different immobilized DNA oligomers and was validated by fluorescence microscopy. Conclusions The immobilization of different types of biomolecules in high-density microarrays is a challenging task for biotechnology. The method presented here not only allows for the deposition of DNA at submicrometer resolution but also for proteins and other molecules of biological relevance that can be coupled to biotin. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 872 KW - Atomic Force Microscope KW - Immobilization KW - Cross Contamination KW - Roth GmbH KW - Microcontact Printing Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-435075 SN - 1866-8372 IS - 872 ER - TY - GEN A1 - Budke, Alexandra T1 - Und der Zukunft abgewandt BT - ideologische Erziehung im Geographieunterricht der DDR T2 - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe N2 - Seit dem Ende der DDR, das den Zusammenbruch des Ostblocks und damit die Beendigung des »Kalten Kriegs« einleitete, wird verstärkt versucht, das Wesen dieses Staates zu definieren und damit seine Folgen auf wirtschaftlicher, sozialer, psychologischer und bildungspolitischer Ebene zu verstehen und einzuordnen. Alexandra Budke analysiert in diesem Band das Schulfach Geographie, das neben der Staatsbürgerkunde und der Geschichte ein zentrales Fach war und in dem die in den Lehrplänen definierte »staatsbürgerliche, weltanschauliche oder ideologische Erziehung« auf der Grundlage des Marxismus-Leninismus stattfinden sollte. Sie klärt, inwiefern Geographieunterricht in der DDR genutzt wurde, um geopolitische Interessen des Staates zu kommunizieren und zu verbreiten. Damit lässt sich durch die detaillierte Analyse des Fachunterrichts auch die Frage beantworten, ob SchülerInnen im Unterricht politisch manipuliert wurden und welche Handlungsmöglichkeiten die zentralen Akteure des Unterrichts, die LehrerInnen und die SchülerInnen, im Rahmen der durch die Bildungspolitik gesetzten curricularen Vorgaben wahrgenommen haben. N2 - Since the end of the German Democratic Republic and with it the collapse of the Eastern Bloc and the termination of the Cold War, efforts have been stepped up to define the real essence of the East German state and thus to understand and classify its consequences in economic, social, psychological and educational terms. In this volume, Alexandra Budke analyses the school subject of geography, which was one of the major subjects besides civics and history, in which “civic, philosophical and ideological education” as defined in the curricula was imparted from a Marxist-Leninist perspective. The author examines to what extent geography lessons in East Germany were used to communicate and propagate the geopolitical interests of the state. Her detailed analysis of geography lessons enables her to assess whether school students were politically manipulated, and what options for action were perceived by the key actors in lessons – namely the teachers and the students – within the scope of the curricula imposed by educational policy. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 813 KW - Deutschland, DDR KW - Ideologische Arbeit KW - Geografieunterricht KW - Geschichte Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-410801 SN - 978-3-89971-627-6 SN - 1866-8372 IS - 813 ER - TY - GEN A1 - Carlsohn, Anja A1 - Cassel, Michael A1 - Linné, Karsten A1 - Mayer, Frank T1 - How much is too much? BT - a case report of nutritional supplement use of a high-performance athlete T2 - Postprints der Universität Potsdam : Humanwissenschaftliche Reihe N2 - Although dietary nutrient intake is often adequate, nutritional supplement use is common among elite athletes. However, high-dose supplements or the use of multiple supplements may exceed the recommended daily allowance (RDA) of particular nutrients or even result in a daily intake above tolerable upper limits (UL). The present case report presents nutritional intake data and supplement use of a highly trained male swimmer competing at international level. Habitual energy and micronutrient intake were analysed by 3 d dietary reports. Supplement use and dosage were assessed, and total amount of nutrient supply was calculated. Micronutrient intake was evaluated based on RDA and UL as presented by the European Scientific Committee on Food, and maximum permitted levels in supplements (MPL) are given. The athlete’s diet provided adequate micronutrient content well above RDA except for vitamin D. Simultaneous use of ten different supplements was reported, resulting in excess intake above tolerable UL for folate, vitamin E and Zn. Additionally, daily supplement dosage was considerably above MPL for nine micronutrients consumed as artificial products. Risks and possible side effects of exceeding UL by the athlete are discussed. Athletes with high energy intake may be at risk of exceeding UL of particular nutrients if multiple supplements are added. Therefore, dietary counselling of athletes should include assessment of habitual diet and nutritional supplement intake. Educating athletes to balance their diets instead of taking supplements might be prudent to prevent health risks that may occur with long-term excess nutrient intake. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 515 KW - dietary supplements KW - tolerable upper limits KW - elite athletes KW - vitamins KW - minerals Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-412910 SN - 1866-8364 IS - 515 ER - TY - GEN A1 - Crome, Erhard T1 - Es geht nicht um "Versagen"! T1 - No "Human Error" N2 - Die sich zunächst um Griechenland rankende Krise wurde zu einer europäischen, die den Euro bedroht. Allenthalben wird die Frage gestellt: Wer ist schuld? In deutschen Groß-Medien wird auf das Zögern der derzeitigen Bundesregierung verwiesen. Der Euro rutschte in die Gefahrenzone. Aber ist der Verweis auf falsche Politik die Antwort auf die Frage nach dem erneuten Aufbrechen der Finanzkrise? KW - Euro KW - Griechenland KW - Weltwirtschaftskrise KW - Euro KW - Greece KW - Global Economic Crisis Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44386 ER - TY - GEN A1 - Dortay, Hakan A1 - Müller-Röber, Bernd T1 - A highly efficient pipeline for protein expression in Leishmania tarentolae sing infrared fluorescence protein as marker N2 - Background: Leishmania tarentolae, a unicellular eukaryotic protozoan, has been established as a novel host for recombinant protein production in recent years. Current protocols for protein expression in Leishmania are, however, time consuming and require extensive lab work in order to identify well-expressing cell lines. Here we established an alternative protein expression work-flow that employs recently engineered infrared fluorescence protein (IFP) as a suitable and easy-to-handle reporter protein for recombinant protein expression in Leishmania. As model proteins we tested three proteins from the plant Arabidopsis thaliana, including a NAC and a type-B ARR transcription factor. Results: IFP and IFP fusion proteins were expressed in Leishmania and rapidly detected in cells by deconvolution microscopy and in culture by infrared imaging of 96-well microtiter plates using small cell culture volumes (2 μL - 100 μL). Motility, shape and growth of Leishmania cells were not impaired by intracellular accumulation of IFP. In-cell detection of IFP and IFP fusion proteins was straightforward already at the beginning of the expression pipeline and thus allowed early pre-selection of well-expressing Leishmania clones. Furthermore, IFP fusion proteins retained infrared fluorescence after electrophoresis in denaturing SDS-polyacrylamide gels, allowing direct in-gel detection without the need to disassemble cast protein gels. Thus, parameters for scaling up protein production and streamlining purification routes can be easily optimized when employing IFP as reporter. Conclusions: Using IFP as biosensor we devised a protocol for rapid and convenient protein expression in Leishmania tarentolae. Our expression pipeline is superior to previously established methods in that it significantly reduces the hands-on-time and work load required for identifying well-expressing clones, refining protein production parameters and establishing purification protocols. The facile in-cell and in-gel detection tools built on IFP make Leishmania amenable for high-throughput expression of proteins from plant and animal sources. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 122 KW - System KW - Donovani Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44773 ER - TY - GEN A1 - Eckstein, Lars T1 - M.I.A.’s “Born Free” and the ambivalent politics of authenticity and provocation T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 83 Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-59236 ER - TY - GEN A1 - Eggert, Sandy T1 - ERP-Architekturen T3 - Zweitveröffentlichungen der Universität Potsdam : Wirtschafts- und Sozialwissenschaftliche Reihe - paper 020 Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44481 ER - TY - GEN A1 - Eggert, Sandy A1 - Meier, Juliane T1 - Business Intelligence : Lösungen im Überblick T3 - Zweitveröffentlichungen der Universität Potsdam : Wirtschafts- und Sozialwissenschaftliche Reihe - paper 019 KW - Business Intelligence KW - Data Mining KW - Reporting KW - Marktrecherche Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44493 ER - TY - GEN A1 - Fanselow, Gisbert A1 - Lenertová, Denisa T1 - Left peripheral focus BT - mismatches between syntax and information structure T2 - Humanwissenschaftliche Reihe N2 - In Czech, German, and many other languages, part of the semantic focus of the utterance can be moved to the left periphery of the clause. The main generalization is that only the leftmost accented part of the semantic focus can be moved. We propose that movement to the left periphery is generally triggered by an unspecific edge feature of C (Chomsky 2008) and its restrictions can be attributed to requirements of cyclic linearization, modifying the theory of cyclic linearization developed by Fox and Pesetsky (2005). The crucial assumption is that structural accent is a direct consequence of being linearized at merge, thus it is indirectly relevant for (locality restrictions on) movement. The absence of structural accent correlates with given-ness. Given elements may later receive (topic or contrastive) accents, which accounts for fronting in multiple focus/contrastive topic constructions. Without any additional assumptions, the model can account for movement of pragmatically unmarked elements to the left periphery (‘formal fronting’, Frey 2005). Crucially, the analysis makes no reference at all to concepts of information structure in the syntax, in line with the claim of Chomsky (2008) that UG specifies no direct link between syntax and information structure. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 596 KW - Czech KW - German KW - Focus KW - Topic KW - Information structure KW - Intervention effects KW - Cyclic linearization KW - A-bar-movement KW - Prosody-syntax interface KW - Accentuation Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-428198 SN - 1866-8364 IS - 596 ER -