TY  - JOUR
A1  - Secker, Christian
A1  - Brosnan, Sarah M.
A1  - Limberg, Felix Rolf Paul
A1  - Braun, Ulrike
A1  - Trunk, Matthias
A1  - Strauch, Peter
A1  - Schlaad, Helmut
T1  - Thermally Induced Crosslinking of Poly(N-Propargyl Glycine)
JF  - Macromolecular chemistry and physics
N2  - As polypeptoids become increasingly popular, they present a more soluble and processable alternative to natural and synthetic polypeptides; the breadth of their potential functionality slowly comes into focus. This report analyzes the ability of an alkyne-functionalized polypeptoid, poly(N-propargyl glycine), to crosslink upon heating. The crosslinking process is analyzed by thermal analysis (differential scanning calorimetry and thermogravimetric analysis), Fourier-transform infrared, electron paramagnetic resonance, and solid-state NMR spectroscopy. While a precise mechanism cannot be confidently assigned, it is clear that the reaction proceeds by a radical mechanism that exclusively involves the alkyne functionality, which, upon crosslinking, yields alkene and aromatic products.
KW  - Fourier-transform infrared
KW  - metal-free crosslinking
KW  - polypeptoid
KW  - propargyl
KW  - solid-state NMR
Y1  - 2015
U6  - https://doi.org/10.1002/macp.201500223
SN  - 1022-1352
SN  - 1521-3935
VL  - 216
IS  - 21
SP  - 2080
EP  - 2085
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Koshkina, Olga
A1  - Westmeier, Dana
A1  - Lang, Thomas
A1  - Bantz, Christoph
A1  - Hahlbrock, Angelina
A1  - Würth, Christian
A1  - Resch-Genger, Ute
A1  - Braun, Ulrike
A1  - Thiermann, Raphael
A1  - Weise, Christoph
A1  - Eravci, Murat
A1  - Mohr, Benjamin
A1  - Schlaad, Helmut
A1  - Stauber, Roland H.
A1  - Docter, Dominic
A1  - Bertin, Annabelle
A1  - Maskos, Michael
T1  - Tuning the Surface of Nanoparticles: Impact of Poly(2-ethyl-2-oxazoline) on Protein Adsorption in Serum and Cellular Uptake
JF  - Macromolecular bioscience
N2  - Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.
KW  - cellular uptake
KW  - nanoparticles
KW  - poly(2-ethyl-2oxazoline)
KW  - poly(ethylene glycol)
KW  - protein adsorption
Y1  - 2016
U6  - https://doi.org/10.1002/mabi.201600074
SN  - 1616-5187
SN  - 1616-5195
VL  - 16
SP  - 1287
EP  - 1300
PB  - Wiley-VCH
CY  - Weinheim
ER  -