TY  - JOUR
A1  - Ribar, Anita
A1  - Huber, Stefan E.
A1  - Smialek, Malgorzata A.
A1  - Tanzer, Katrin
A1  - Neustetter, Michael
A1  - Schürmann, Robin
A1  - Bald, Ilko
A1  - Denifl, Stephan
T1  - Hydroperoxyl radical and formic acid formation from common DNA stabilizers upon low energy electron attachment
JF  - Physical chemistry, chemical physics : a journal of European Chemical Societies
N2  - 2-Amino-2-(hydroxymethyl)-1,3-propanediol (TRIS) and ethylenediaminetetraacetic acid ( EDTA) are key components of biological buffers and are frequently used as DNA stabilizers in irradiation studies. Such surface or liquid phase studies are done with the aim to understand the fundamental mechanisms of DNA radiation damage and to improve cancer radiotherapy. When ionizing radiation is used, abundant secondary electrons are formed during the irradiation process, which are able to attach to the molecular compounds present on the surface. In the present study we experimentally investigate low energy electron attachment to TRIS and methyliminodiacetic acid ( MIDA), an analogue of EDTA, supported by quantum chemical calculations. The most prominent dissociation channel for TRIS is through hydroperoxyl radical formation, whereas the dissociation of MIDA results in the formation of formic and acetic acid. These compounds are well-known to cause DNA modifications, like strand breaks. The present results indicate that buffer compounds may not have an exclusive protecting effect on DNA as suggested previously.
Y1  - 2018
U6  - https://doi.org/10.1039/c7cp07697e
SN  - 1463-9076
SN  - 1463-9084
VL  - 20
IS  - 8
SP  - 5578
EP  - 5585
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  -