TY - JOUR A1 - Jedrusik-Bode, Monika A1 - Studencka, Maja A1 - Smolka, Christian A1 - Baumann, Tobias A1 - Schmidt, Henning A1 - Kampf, Jan A1 - Paap, Franziska A1 - Martin, Sophie A1 - Tazi, Jamal A1 - Müller, Kristian M. A1 - Krüger, Marcus A1 - Braun, Thomas A1 - Bober, Eva T1 - The sirtuin SIRT6 regulates stress granule formation in C. elegans and mammals JF - Journal of cell science N2 - SIRT6 is a NAD(+)-dependent deacetylase that modulates chromatin structure and safeguards genomic stability. Until now, SIRT6 has been assigned to the nucleus and only nuclear targets of SIRT6 are known. Here, we demonstrate that in response to stress, C. elegans SIR-2.4 and its mammalian orthologue SIRT6 localize to cytoplasmic stress granules, interact with various stress granule components and induce their assembly. Loss of SIRT6 or inhibition of its catalytic activity in mouse embryonic fibroblasts impairs stress granule formation and delays disassembly during recovery, whereas deficiency of SIR-2.4 diminishes maintenance of P granules and decreases survival of C. elegans under stress conditions. Our findings uncover a novel, evolutionary conserved function of SIRT6 in the maintenance of stress granules in response to stress. KW - C. elegans KW - G3BP KW - SIRT6 KW - Sirtuins KW - Stress KW - Stress granules Y1 - 2013 U6 - https://doi.org/10.1242/jcs.130708 SN - 0021-9533 SN - 1477-9137 VL - 126 IS - 22 SP - 5166 EP - + PB - Company of Biologists Limited CY - Cambridge ER - TY - JOUR A1 - Cesca, Simone A1 - Braun, Thomas A1 - Maccaferri, Francesco A1 - Passarelli, Luigi A1 - Rivalta, Eleonora A1 - Dahm, Torsten T1 - Source modelling of the M5-6 Emilia-Romagna, Italy, earthquakes (2012 May 20-29) JF - Geophysical journal international N2 - On 2012 May 20 and 29, two damaging earthquakes with magnitudes M-w 6.1 and 5.9, respectively, struck the Emilia-Romagna region in the sedimentary Po Plain, Northern Italy, causing 26 fatalities, significant damage to historical buildings and substantial impact to the economy of the region. The earthquake sequence included four more aftershocks with M-w, >= 5.0, all at shallow depths (about 7-9 km), with similar WNW-ESE striking reverse mechanism. The timeline of the sequence suggests significant static stress interaction between the largest events. We perform here a detailed source inversion, first adopting a point source approximation and considering pure double couple and full moment tensor source models. We compare different extended source inversion approaches for the two largest events, and find that the rupture occurred in both cases along a subhorizontal plane, dipping towards SSW Directivity is well detected for the May 20 main shock, indicating that the rupture propagated unilaterally towards SE. Based on the focal mechanism solution, we further estimate the co-seismic static stress change induced by the May 20 event. By using the rate-and-state model and a Poissonian earthquake occurrence, we infer that the second largest event of May 29 was induced with a probability in the range 0.2-0.4. This suggests that the segment of fault was already prone to rupture. Finally, we estimate peak ground accelerations for the two main events as occurred separately or simultaneously. For the scenario involving hypothetical rupture areas of both main events, we estimate M-w = 6.3 and an increase of ground acceleration by 50 per cent. The approach we propose may help to quantify rapidly which regions are invested by a significant increase of the hazard, bearing the potential for large aftershocks or even a second main shock. KW - Earthquake dynamics KW - Earthquake source observations Y1 - 2013 U6 - https://doi.org/10.1093/gji/ggt069 SN - 0956-540X VL - 193 IS - 3 SP - 1658 EP - 1672 PB - Oxford Univ. Press CY - Oxford ER - TY - JOUR A1 - Dahm, Torsten A1 - Cesca, Simone A1 - Hainzl, Sebastian A1 - Braun, Thomas A1 - Krüger, Frank T1 - Discrimination between induced, triggered, and natural earthquakes close to hydrocarbon reservoirs: A probabilistic approach based on the modeling of depletion-induced stress changes and seismological source parameters JF - Journal of geophysical research : Solid earth N2 - Earthquakes occurring close to hydrocarbon fields under production are often under critical view of being induced or triggered. However, clear and testable rules to discriminate the different events have rarely been developed and tested. The unresolved scientific problem may lead to lengthy public disputes with unpredictable impact on the local acceptance of the exploitation and field operations. We propose a quantitative approach to discriminate induced, triggered, and natural earthquakes, which is based on testable input parameters. Maxima of occurrence probabilities are compared for the cases under question, and a single probability of being triggered or induced is reported. The uncertainties of earthquake location and other input parameters are considered in terms of the integration over probability density functions. The probability that events have been human triggered/induced is derived from the modeling of Coulomb stress changes and a rate and state-dependent seismicity model. In our case a 3-D boundary element method has been adapted for the nuclei of strain approach to estimate the stress changes outside the reservoir, which are related to pore pressure changes in the field formation. The predicted rate of natural earthquakes is either derived from the background seismicity or, in case of rare events, from an estimate of the tectonic stress rate. Instrumentally derived seismological information on the event location, source mechanism, and the size of the rupture plane is of advantage for the method. If the rupture plane has been estimated, the discrimination between induced or only triggered events is theoretically possible if probability functions are convolved with a rupture fault filter. We apply the approach to three recent main shock events: (1) the M-w 4.3 Ekofisk 2001, North Sea, earthquake close to the Ekofisk oil field; (2) the M-w 4.4 Rotenburg 2004, Northern Germany, earthquake in the vicinity of the Sohlingen gas field; and (3) the M-w 6.1 Emilia 2012, Northern Italy, earthquake in the vicinity of a hydrocarbon reservoir. The three test cases cover the complete range of possible causes: clearly human induced, not even human triggered, and a third case in between both extremes. KW - induced seismicity KW - probabilistic discrimination KW - hydrocarbon field KW - triggered earthquake KW - seismic hazard KW - earthquake Y1 - 2015 U6 - https://doi.org/10.1002/2014JB011778 SN - 2169-9313 SN - 2169-9356 VL - 120 IS - 4 SP - 2491 EP - 2509 PB - American Geophysical Union CY - Washington ER -