TY  - JOUR
A1  - Sulpice, Ronan
A1  - Pyl, Eva-Theresa
A1  - Ishihara, Hirofumi
A1  - Trenkamp, Sandra
A1  - Steinfath, Matthias
A1  - Witucka-Wall, Hanna
A1  - Gibon, Yves
A1  - Usadel, Björn
A1  - Poree, Fabien
A1  - Piques, Maria Conceicao
A1  - von Korff, Maria
A1  - Steinhauser, Marie Caroline
A1  - Keurentjes, Joost J. B.
A1  - Guenther, Manuela
A1  - Hoehne, Melanie
A1  - Selbig, Joachim
A1  - Fernie, Alisdair R.
A1  - Altmann, Thomas
A1  - Stitt, Mark
T1  - Starch as a major integrator in the regulation of plant growth
N2  - Rising demand for food and bioenergy makes it imperative to breed for increased crop yield. Vegetative plant growth could be driven by resource acquisition or developmental programs. Metabolite profiling in 94 Arabidopsis accessions revealed that biomass correlates negatively with many metabolites, especially starch. Starch accumulates in the light and is degraded at night to provide a sustained supply of carbon for growth. Multivariate analysis revealed that starch is an integrator of the overall metabolic response. We hypothesized that this reflects variation in a regulatory network that balances growth with the carbon supply. Transcript profiling in 21 accessions revealed coordinated changes of transcripts of more than 70 carbon-regulated genes and identified 2 genes (myo-inositol-1- phosphate synthase, a Kelch-domain protein) whose transcripts correlate with biomass. The impact of allelic variation at these 2 loci was shown by association mapping, identifying them as candidate lead genes with the potential to increase biomass production.
Y1  - 2009
UR  - http://www.pnas.org/
U6  - https://doi.org/10.1073/pnas.0903478106
SN  - 0027-8424
ER  - 
TY  - JOUR
A1  - Küken, Anika
A1  - Sommer, Frederik
A1  - Yaneva-Roder, Liliya
A1  - Mackinder, Luke C. M.
A1  - Hoehne, Melanie
A1  - Geimer, Stefan
A1  - Jonikas, Martin C.
A1  - Schroda, Michael
A1  - Stitt, Mark
A1  - Nikoloski, Zoran
A1  - Mettler-Altmann, Tabea
T1  - Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts
JF  - eLife
N2  - Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concentrating mechanism (CCM) that improves the photosynthetic performance of eukaryotic algae. Using Chlamydomonas reinhardtii, we obtained experimental data on photosynthesis, metabolites, and proteins in CCM-induced and CCM-suppressed cells. We then employed a computational strategy to estimate how fluxes through the Calvin-Benson cycle are compartmented between the pyrenoid and the stroma. Our model predicts that ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco, and 3-phosphoglycerate (3PGA), its product, diffuse in and out of the pyrenoid, respectively, with higher fluxes in CCM-induced cells. It also indicates that there is no major diffusional barrier to metabolic flux between the pyrenoid and stroma. Our computational approach represents a stepping stone to understanding microcompartmentalized CCM in other organisms.
Y1  - 2018
U6  - https://doi.org/10.7554/eLife.37960
SN  - 2050-084X
VL  - 7
PB  - eLife Sciences Publications
CY  - Cambridge
ER  -