TY  - JOUR
A1  - Beissenhirtz, Moritz Karl
A1  - Scheller, Frieder W.
A1  - Lisdat, Fred
T1  - Immobilized cytochrome c sensor in organic / aqueous media for the characterization of hydrophilic and hydrophobic antioxidants
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Büttemeyer, R.
A1  - Philipp, A. W
A1  - Schlenzka, L.
A1  - Mall, J. W.
A1  - Beissenhirtz, Moritz Karl
A1  - Lisdat, Fred
T1  - Epigallocatechin gallate can significantly decrease free oxygen radicals in the reperfusion insury in vivo
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Ge, Bixia
A1  - Scheller, Frieder W.
A1  - Lisdat, Fred
T1  - Electrochemistry of immobilized CuZnSOD and FeSOD and their interaction with superoxide radicals
N2  - Copper, zinc superoxide dismutase (CuZnSOD) from bovine erythrocytes and iron superoxide dismutase from Escherichia coli (FeSOD) were immobilized on 3-mercaptopropionic acid (MPA)-modified gold electrodes, respectively. The characterization of the SOD electrodes showed a quasi-reversible, electrochemical redox behavior with a formal potential of 47 ñ 4 mV and -154 ñ 5 mV (vs. Ag/AgCl, 1 M KCl) for surface adsorbed CuZnSOD and FeSOD, respectively. The heterogeneous electron transfer rate constants were determined to be about 65 and 35/s, respectively. Covalent fixation of both SODs was also feasible with only slight changes in the formal potential. The interaction of superoxide radicals (O2-) with the SOD electrode was investigated. No catalytic current could be observed. However, due to the fast cyclic reaction of SOD with superoxide, the communication of the protein with the electrode was strongly influenced. The amperometric detection of superoxide radicals is discussed.
Y1  - 2003
ER  -