TY  - JOUR
A1  - Annunziata, Maria Grazia
A1  - Apelt, Federico
A1  - Carillo, Petronia
A1  - Krause, Ursula
A1  - Feil, Regina
A1  - Mengin, Virginie
A1  - Lauxmann, Martin A.
A1  - Koehl, Karin
A1  - Nikoloski, Zoran
A1  - Stitt, Mark
A1  - Lunn, John Edward
T1  - Getting back to nature: a reality check for experiments in controlled environments
JF  - Journal of experimental botany
N2  - Irradiance from sunlight changes in a sinusoidal manner during the day, with irregular fluctuations due to clouds, and light-dark shifts at dawn and dusk are gradual. Experiments in controlled environments typically expose plants to constant irradiance during the day and abrupt light-dark transitions. To compare the effects on metabolism of sunlight versus artificial light regimes, Arabidopsis thaliana plants were grown in a naturally illuminated greenhouse around the vernal equinox, and in controlled environment chambers with a 12-h photoperiod and either constant or sinusoidal light profiles, using either white fluorescent tubes or light-emitting diodes (LEDs) tuned to a sunlight-like spectrum as the light source. Rosettes were sampled throughout a 24-h diurnal cycle for metabolite analysis. The diurnal metabolite profiles revealed that carbon and nitrogen metabolism differed significantly between sunlight and artificial light conditions. The variability of sunlight within and between days could be a factor underlying these differences. Pairwise comparisons of the artificial light sources (fluorescent versus LED) or the light profiles (constant versus sinusoidal) showed much smaller differences. The data indicate that energy-efficient LED lighting is an acceptable alternative to fluorescent lights, but results obtained from plants grown with either type of artificial lighting might not be representative of natural conditions.
KW  - Amino acid
KW  - Arabidopsis thaliana
KW  - controlled environment
KW  - LED lighting
KW  - visible light spectrum
KW  - organic acid
KW  - starch
KW  - sucrose
KW  - trehalose 6-phosphate
Y1  - 2017
U6  - https://doi.org/10.1093/jxb/erx220
SN  - 0022-0957
SN  - 1460-2431
VL  - 68
SP  - 4463
EP  - 4477
PB  - Oxford Univ. Press
CY  - Oxford
ER  -