TY  - GEN
A1  - Luckner, Madlen
A1  - Dunsing, Valentin
A1  - Drüke, Markus
A1  - Zuehlke, B.
A1  - Petazzi, Roberto Arturo
A1  - Chiantia, Salvatore
A1  - Herrmann, A.
T1  - Quantifying protein oligomerization directly in living cells
BT  - a systematic comparison of fluorescent proteins and application to Influenza A virus infection
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2019
SN  - 0175-7571
SN  - 1432-1017
VL  - 48
SP  - S183
EP  - S183
PB  - Springer
CY  - New York
ER  - 
TY  - JOUR
A1  - Dunsing, Valentin
A1  - Luckner, Madlen
A1  - Zuehlke, Boris
A1  - Petazzi, Roberto Arturo
A1  - Herrmann, Andreas
A1  - Chiantia, Salvatore
T1  - Optimal fluorescent protein tags for quantifying protein oligomerization in living cells
JF  - Scientific reports
N2  - Fluorescence fluctuation spectroscopy has become a popular toolbox for non-disruptive analysis of molecular interactions in living cells. The quantification of protein oligomerization in the native cellular environment is highly relevant for a detailed understanding of complex biological processes. An important parameter in this context is the molecular brightness, which serves as a direct measure of oligomerization and can be easily extracted from temporal or spatial fluorescence fluctuations. However, fluorescent proteins (FPs) typically used in such studies suffer from complex photophysical transitions and limited maturation, inducing non-fluorescent states. Here, we show how these processes strongly affect molecular brightness measurements. We perform a systematic characterization of non-fluorescent states for commonly used FPs and provide a simple guideline for accurate, unbiased oligomerization measurements in living cells. Further, we focus on novel red FPs and demonstrate that mCherry2, an mCherry variant, possesses superior properties with regards to precise quantification of oligomerization.
Y1  - 2018
U6  - https://doi.org/10.1038/s41598-018-28858-0
SN  - 2045-2322
VL  - 8
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - GEN
A1  - Luckner, Madlen
A1  - Dunsing, Valentin
A1  - Chiantia, Salvatore
A1  - Hermann, Andreas
T1  - Oligomerization and nuclear shuttling dynamics of viral proteins studied by quantitative molecular brightness analysis using fluorescence correlation spectroscopy
T2  - Biophysical journal
Y1  - 2018
U6  - https://doi.org/10.1016/j.bpj.2017.11.1951
SN  - 0006-3495
SN  - 1542-0086
VL  - 114
IS  - 3
SP  - 350A
EP  - 350A
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - GEN
A1  - Luckner, Madlen
A1  - Dunsing, Valentin
A1  - Chiantia, Salvatore
A1  - Herrmann, Andreas
T1  - Influenza virus vRNPs: quantitative investigations via fluorescence
cross-correlation spectroscopy
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2017
SN  - 0175-7571
SN  - 1432-1017
VL  - 46
SP  - S368
EP  - S368
PB  - Springer
CY  - New York
ER  -