TY  - THES
A1  - Grießner, Matthias
T1  - Grenzflächenmodifizierung von Mikrosystemen für biochemische Assays
Y1  - 2011
CY  - Potsdam
ER  - 
TY  - JOUR
A1  - Grießner, Matthias
A1  - Broeker, Patrick
A1  - Lehmann, André
A1  - Ehrentreich-Förster, Eva
A1  - Bier, Frank Fabian
T1  - Detection of angiotensin II type 1 receptor ligands by a cell-based assay
N2  - This work describes a cell-based assay that does not depend on radioactivity or laboratory animals for the detection of ligands of angiotensin II type 1 receptor (AT(1)R). The assay makes use of stable transfected Chinese hamster ovary cells (CHO-AT(1)R) expressing the AT(1)R. A sequential saturation assay principle was used in which receptor binding sites of the CHO-AT(1)R cells are blocked by the analyte in a concentration-dependent manner. Afterwards, TAMRA-angiotensin II, a fluorescence-labeled ligand, was added to bind to the remaining free binding sites of the receptor. In consequence, the fluorescence signal determined is inversely proportional to the concentration of the analyte.
Y1  - 2009
UR  - http://www.springerlink.com/content/100417
U6  - https://doi.org/10.1007/s00216-009-3074-4
SN  - 1618-2642
ER  - 
TY  - JOUR
A1  - Grießner, Matthias
A1  - Hartig, Dave
A1  - Christmann, Alexander
A1  - Ehrentreich-Förster, Eva
A1  - Warsinke, Axel
A1  - Bier, Frank Fabian
T1  - Surface regeneration of microfluidic microarray printheads through plasma techniques
N2  - This work describes a method for surface regeneration of microfluidic microarray printheads through plasma techniques. Modification procedures were chosen in a way to obtain high reproducibility with a minimum of time consumption. The idea behind this is a complete regeneration of a microarray printhead before or after usage to achieve best printing results over a typical print job. A sequence of low-pressure oxygen-plasma and plasma polymerization with hexamethyldisiloxane (HMDSO) was used to regenerate printheads. Proof of the concept is given through quality control performed with a spotter implemented CCD camera, contact angle measurements and a typical hybridization experiment. Stable printing results were obtained over 3000 activations showing that the presented method is suitable for treatment of microarray printheads.
Y1  - 2010
UR  - http://iopscience.iop.org/0960-1317/
U6  - https://doi.org/10.1088/0960-1317/20/3/037002
SN  - 0960-1317
ER  -