TY  - JOUR
A1  - Damaraju, Sridevi
A1  - Schlede, Stephanie
A1  - Eckhardt, Ulrich
A1  - Lokstein, Heiko
A1  - Grimm, Bernhard
T1  - Functions of the water soluble chlorophyll-binding protein in plants
JF  - Journal of plant physiology : biochemistry, physiology, molecular biology and biotechnology of plants
N2  - Functional aspects of water soluble chlorophyll-binding protein (WSCP) in plants were investigated during the courses of leaf senescence, chlorophyll biogenesis, stress response and photoprotection. The cDNA sequence encoding WSCP from cauliflower was cloned into a binary vector to facilitate Agrobacterium tumefaciens mediated transformation of Nicotiana tabacum. The resultant transgenic tobacco plants overexpressed the CauWSCP gene under the control of a 35S-promoter. Analyses of protein and pigment contents indicate that WSCP overexpression does not enhance chlorophyll catabolism in vivo, thus rendering a role of WSCP in Chl degradation unlikely. Accumulation of higher levels of protochlorophyllide in WSCP overexpressor plants corroborates a proposed temporary storage and carrier function of WSCP for chlorophyll and late precursors. Although WSCP overexpressor plants did not show significant differences in non-photochemical quenching of chlorophyll fluorescence, they are characterized by significantly lower zeaxanthin accumulation and peroxidase activity at different light intensities, even at high light intensities of 700-900 mu mol photons m(-2) s(-1). These results suggest a photoprotective function of the functional chlorophyll binding-WSCP tetramer by shielding of chlorophylls from molecular oxygen.
KW  - Chlorophyll metabolism
KW  - Non-photochemical quenching of chlorophyll fluorescence
KW  - Photooxidation
KW  - Photoprotection
KW  - Photosynthesis
Y1  - 2011
U6  - https://doi.org/10.1016/j.jplph.2011.02.007
SN  - 0176-1617
VL  - 168
IS  - 12
SP  - 1444
EP  - 1451
PB  - Elsevier
CY  - Jena
ER  -