TY  - JOUR
A1  - Dauvillee, David
A1  - Chochois, Vincent
A1  - Steup, Martin
A1  - Haebel, Sophie
A1  - Eckermann, Nora
A1  - Ritte, Gerhard
A1  - Ral, Jean-Philippe
A1  - Colleoni, Christophe
A1  - Hicks, Glenn
A1  - Wattebled, Fabrice
A1  - Deschamps, Philippe
A1  - Lienard, Luc
A1  - Cournac, Laurent
A1  - Putaux, Jean-Luc
A1  - Dupeyre, Danielle
A1  - Ball, Steven G.
T1  - Plastidial phosphorylase is required for normal starch synthesis in Chlamydomonas reinhardtii
JF  - The plant journal
N2  - Among the three distinct starch phosphorylase activities detected in Chlamydomonas reinhardtii, two distinct plastidial enzymes (PhoA and PhoB) are documented while a single extraplastidial form (PhoC) displays a higher affinity for glycogen as in vascular plants. The two plastidial phosphorylases are shown to function as homodimers containing two 91-kDa (PhoA) subunits and two 110-kDa (PhoB) subunits. Both lack the typical 80-amino-acid insertion found in the higher plant plastidial forms. PhoB is exquisitely sensitive to inhibition by ADP-glucose and has a low affinity for malto-oligosaccharides. PhoA is more similar to the higher plant plastidial phosphorylases: it is moderately sensitive to ADP-glucose inhibition and has a high affinity for unbranched malto-oligosaccharides. Molecular analysis establishes that STA4 encodes PhoB. Chlamydomonas reinhardtii strains carrying mutations at the STA4 locus display a significant decrease in amounts of starch during storage that correlates with the accumulation of abnormally shaped granules containing a modified amylopectin structure and a high amylose content. The wild-type phenotype could be rescued by reintroduction of the cloned wild-type genomic DNA, thereby demonstrating the involvement of phosphorylase in storage starch synthesis.
KW  - Chlamydomonas
KW  - starch
KW  - amylopectin
KW  - (glycogen) starch phosphorylase
Y1  - 2006
U6  - https://doi.org/10.1111/j.1365-313X.2006.02870.x
SN  - 0960-7412
VL  - 48
IS  - 2
SP  - 274
EP  - 285
PB  - Blackwell
CY  - Oxford
ER  -