TY  - JOUR
A1  - Wand, Inga
A1  - Holzlöhner, Pamela
A1  - Neupert, Steffi
A1  - Micheel, Burkhard
A1  - Heilmann, Katja
T1  - Cooperation of dendritic cells with naive lymphocyte populations to induce the generation of antigen-specific antibodies in vitro
JF  - Journal of biotechnology
N2  - The production of monoclonal antibodies by hybridoma technology is dependent on lymphocytes taken from vertebrates which have to be immunized against the corresponding antigen. We present here our first experiments which should allow the replacement of this in vivo immunization step by an in vitro immunization procedure. This work provides new possibilities for the specific activation of immune cells in order to use them for the generation of antibodies which are not of murine origin. Bone marrow-derived dendritic cells were loaded with antigen and co-cultured with naive T and B lymphocytes of non-immunized mice. The interaction and activation of the different cell types were investigated by measuring the expression of specific cell surface markers, the release of activation-dependent interleukins and the secretion of antigen-specific antibodies. We could demonstrate that dendritic cells process and present antigen fragments and activate T cells, that T cells proliferate and release activation-induced interleukins, and that B cells maturate under the influence of activated T cells and secrete antigen-specific antibodies.
KW  - In vitro immunization
KW  - Activation of dendritic cells
KW  - Interaction of T and B cells with antigen-presenting cells
KW  - Induction of antibody responses
Y1  - 2011
U6  - https://doi.org/10.1016/j.jbiotec.2011.09.002
SN  - 0168-1656
VL  - 156
IS  - 3
SP  - 173
EP  - 181
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Heilmann, Katja
A1  - Wand, Inga
A1  - Holzlöhner, Pamela
A1  - Micheel, Burkhard
T1  - Cooperation of dendritic cells with naive lymphocyte populations to induce the generation of antigen-specific antibodies in vitro
T2  - The journal of immunology
Y1  - 2012
SN  - 0022-1767
VL  - 188
IS  - 6
PB  - American Assoc. of Immunologists
CY  - Bethesda
ER  -