TY  - JOUR
A1  - Hilson, Pierre
A1  - Allemeersch, Joke
A1  - Altmann, Thomas
A1  - Aubourg, Sebastien
A1  - Avon, Alexandra
A1  - Beynon, Jim
A1  - Bhalerao, Rishikesh P.
A1  - Bitton, Frederique
A1  - Caboche, Michel
A1  - Cannoot, Bernard
A1  - Chardakov, Vasil
A1  - Cognet-Holliger, Cecile
A1  - Colot, Vincent
A1  - Crowe, Mark
A1  - Darimont, Caroline
A1  - Durinck, Steffen
A1  - Eickhoff, Holger
A1  - deLongevialle, Andeol Falcon
A1  - Farmer, Edward E.
A1  - Grant, Murray
A1  - Kuiper, Martin T. R.
A1  - Lehrach, Hans
A1  - Leon, Celine
A1  - Leyva, Antonio
A1  - Lundeberg, Joakim
A1  - Lurin, Claire
A1  - Moreau, Yves
T1  - Versatile gene-specific sequence tags for arabidopsis functional genomics : transcript profiling and reserve genetics applications
N2  - Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Gendre, Delphine
A1  - Baral, Anirban
A1  - Dang, Xie
A1  - Esnay, Nicolas
A1  - Boutte, Yohann
A1  - Stanislas, Thomas
A1  - Vain, Thomas
A1  - Claverol, Stephane
A1  - Gustavsson, Anna
A1  - Lin, Deshu
A1  - Grebe, Markus
A1  - Bhalerao, Rishikesh P.
T1  - Rho-of-plant activated root hair formation requires Arabidopsis YIP4a/b gene function
JF  - Development : Company of Biologists
N2  - Root hairs are protrusions from root epidermal cells with crucial roles in plant soil interactions. Although much is known about patterning, polarity and tip growth of root hairs, contributions of membrane trafficking to hair initiation remain poorly understood. Here, we demonstrate that the trans-Golgi network-localized YPT-INTERACTING PROTEIN 4a and YPT-INTERACTING PROTEIN 4b (YIP4a/b) contribute to activation and plasma membrane accumulation of Rho-of-plant (ROP) small GTPases during hair initiation, identifying YIP4a/b as central trafficking components in ROP-dependent root hair formation.
KW  - ROP
KW  - YIP
KW  - Root hair
KW  - Secretion
KW  - Trans-Golgi network
Y1  - 2019
U6  - https://doi.org/10.1242/dev.168559
SN  - 0950-1991
SN  - 1477-9129
VL  - 146
IS  - 5
PB  - The Company of Biologists
CY  - Cambridge
ER  -