TY  - JOUR
A1  - Nawaz, Shiza
A1  - Khan, Muhammad Moman
A1  - Noack, Jonas
A1  - Awan, Asad Bashir
A1  - Schiebel, Juliane
A1  - Roggenbuck, Dirk
A1  - Schierack, Peter
A1  - Sarwar, Yasra
A1  - Ali, Aamir
T1  - Rapid detection of biofilm formation by zoonotic serovars of Salmonella enterica and avian pathogenic E. coli isolates from poultry
JF  - Pakistan veterinary journal
N2  - Biofilms are complex, sessile microbial communities that are problematic in clinical settings due to their association with survival and pathogenicity of bacteria. The biofilm formation supporting conditions for zoonotic serovars of Salmonella and avian pathogenic E. coli (APEC) from poultry have not been well studied yet. Clinical isolates of zoonotic Salmonella and APEC from poultry were evaluated for biofilm formation in four media at 37 degrees C and 40 degrees C after incubation of 48 and 72 hrs. The biofilms formed in 96 well plates were visualized and quantified with a new module of Aklides system using fluorescence microscope coupled with automated VideoScan Technology. After 72 hrs, brain heart infusion at 40 degrees C and Rappaport-Vassiliadis Soya broth at 37 degrees C were found most suitable for APEC and Salmonella biofilm formations respectively. The new information will be useful for further biofilm associated studies particularly for evaluation of antibiofilm compounds and contribute in infection control. (C) 2020 PVJ. All rights reserved
KW  - APEC
KW  - biofilm formation
KW  - Salmonella
KW  - video scan technology
Y1  - 2020
U6  - https://doi.org/10.29261/pakvetj/2020.066
SN  - 0253-8318
SN  - 2074-7764
VL  - 40
IS  - 4
SP  - 527
EP  - 530
PB  - University of Agriculture, Faculty of Veterinary Science
CY  - Faisalabad
ER  -