TY  - JOUR
A1  - Bordag, Natalie
A1  - Klie, Sebastian
A1  - Jürchott, Kathrin
A1  - Vierheller, Janine
A1  - Schiewe, Hajo
A1  - Albrecht, Valerie
A1  - Tonn, Jörg-Christian
A1  - Schwartz, Christoph
A1  - Schichor, Christian
A1  - Selbig, Joachim
T1  - Glucocorticoid (dexamethasone)-induced metabolome changes in healthy males suggest prediction of response and side effects
JF  - Scientific reports
N2  - Glucocorticoids are indispensable anti-inflammatory and decongestant drugs with high prevalence of use at (similar to)0.9% of the adult population. Better holistic insights into glucocorticoid-induced changes are crucial for effective use as concurrent medication and management of adverse effects. The profiles of 214 metabolites from plasma of 20 male healthy volunteers were recorded prior to and after ingestion of a single dose of 4 mg dexamethasone (+20 mg pantoprazole). Samples were drawn at three predefined time points per day: seven untreated (day 1 midday - day 3 midday) and four treated (day 3 evening - day 4 evening) per volunteer. Statistical analysis revealed tremendous impact of dexamethasone on the metabolome with 150 of 214 metabolites being significantly deregulated on at least one time point after treatment (ANOVA, Benjamini-Hochberg corrected, q < 0.05). Inter-person variability was high and remained uninfluenced by treatment. The clearly visible circadian rhythm prior to treatment was almost completely suppressed and deregulated by dexamethasone. The results draw a holistic picture of the severe metabolic deregulation induced by single-dose, short-term glucocorticoid application. The observed metabolic changes suggest a potential for early detection of severe side effects, raising hope for personalized early countermeasures increasing quality of life and reducing health care costs.
Y1  - 2015
U6  - https://doi.org/10.1038/srep15954
SN  - 2045-2322
VL  - 5
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Omranian, Nooshin
A1  - Kleessen, Sabrina
A1  - Tohge, Takayuki
A1  - Klie, Sebastian
A1  - Basler, Georg
A1  - Müller-Röber, Bernd
A1  - Fernie, Alisdair R.
A1  - Nikoloski, Zoran
T1  - Differential metabolic and coexpression networks of plant metabolism
JF  - Trends in plant science
N2  - Recent analyses have demonstrated that plant metabolic networks do not differ in their structural properties and that genes involved in basic metabolic processes show smaller coexpression than genes involved in specialized metabolism. By contrast, our analysis reveals differences in the structure of plant metabolic networks and patterns of coexpression for genes in (non)specialized metabolism. Here we caution that conclusions concerning the organization of plant metabolism based on network-driven analyses strongly depend on the computational approaches used.
KW  - plant specialized metabolism
KW  - metabolic networks
KW  - gene coexpression
KW  - differential network analysis
Y1  - 2015
U6  - https://doi.org/10.1016/j.tplants.2015.02.002
SN  - 1360-1385
VL  - 20
IS  - 5
SP  - 266
EP  - 268
PB  - Elsevier
CY  - London
ER  -