TY  - JOUR
A1  - Bemer, Marian
A1  - van Mourik, Hilda
A1  - Muino, Jose M.
A1  - Ferrandiz, Cristina
A1  - Kaufmann, Kerstin
A1  - Angenent, Gerco C.
T1  - FRUITFULL controls SAUR10 expression and regulates Arabidopsis growth and architecture
JF  - Journal of experimental botany
N2  - MADS-domain transcription factors are well known for their roles in plant development and regulate sets of downstream genes that have been uncovered by high-throughput analyses. A considerable number of these targets are predicted to function in hormone responses or responses to environmental stimuli, suggesting that there is a close link between developmental and environmental regulators of plant growth and development. Here, we show that the Arabidopsis MADS-domain factor FRUITFULL (FUL) executes several functions in addition to its noted role in fruit development. Among the direct targets of FUL, we identified SMALL AUXIN UPREGULATED RNA 10 (SAUR10), a growth regulator that is highly induced by a combination of auxin and brassinosteroids and in response to reduced R:FR light. Interestingly, we discovered that SAUR10 is repressed by FUL in stems and inflorescence branches. SAUR10 is specifically expressed at the abaxial side of these branches and this localized activity is influenced by hormones, light conditions and by FUL, which has an effect on branch angle. Furthermore, we identified a number of other genes involved in hormone pathways and light signalling as direct targets of FUL in the stem, demonstrating a connection between developmentally and environmentally regulated growth programs.
KW  - Architecture
KW  - auxin
KW  - branching
KW  - FRUITFULL
KW  - growth
KW  - hormones
KW  - light response
KW  - MADS-box transcription factor
KW  - SAUR
Y1  - 2017
U6  - https://doi.org/10.1093/jxb/erx184
SN  - 0022-0957
SN  - 1460-2431
VL  - 68
SP  - 3391
EP  - 3403
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Smaczniak, Cezary
A1  - Muino, Jose M.
A1  - Chen, Dijun
A1  - Angenent, Gerco C.
A1  - Kaufmann, Kerstin
T1  - Differences in DNA Binding Specificity of Floral Homeotic Protein Complexes Predict Organ-Specific Target Genes
JF  - The plant cell
N2  - Floral organ identities in plants are specified by the combinatorial action of homeotic master regulatory transcription factors. However, how these factors achieve their regulatory specificities is still largely unclear. Genome-wide in vivo DNA binding data show that homeotic MADS domain proteins recognize partly distinct genomic regions, suggesting that DNA binding specificity contributes to functional differences of homeotic protein complexes. We used in vitro systematic evolution of ligands by exponential enrichment followed by high-throughput DNA sequencing (SELEX-seq) on several floral MADS domain protein homo-and heterodimers to measure their DNA binding specificities. We show that specification of reproductive organs is associated with distinct binding preferences of a complex formed by SEPALLATA3 and AGAMOUS. Binding specificity is further modulated by different binding site spacing preferences. Combination of SELEX-seq and genome-wide DNA binding data allows differentiation between targets in specification of reproductive versus perianth organs in the flower. We validate the importance of DNA binding specificity for organ-specific gene regulation by modulating promoter activity through targeted mutagenesis. Our study shows that intrafamily protein interactions affect DNA binding specificity of floral MADS domain proteins. Differential DNA binding of MADS domain protein complexes plays a role in the specificity of target gene regulation.
Y1  - 2017
U6  - https://doi.org/10.1105/tpc.17.00145
SN  - 1040-4651
SN  - 1532-298X
VL  - 29
SP  - 1822
EP  - 1835
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  -