TY  - JOUR
A1  - Velk, Natalia
A1  - Uhlig, Katja
A1  - Vikulina, Anna
A1  - Duschl, Claus
A1  - Volodkin, Dmitry
T1  - Mobility of lysozyme in poly(L-lysine)/hyaluronic acid multilayer films
JF  - Colloids and surfaces : an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin ; B, Biointerfaces
N2  - The spatial and temporal control over presentation of protein-based biomolecules such as growth factors and hormones is crucial for in vitro applications to mimic the complex in vivo environment. We investigated the interaction of a model protein lysozyme (Lys) with poly(L-lysine)/hyaluronic acid (PLL/HA) multilayer films. We focused on Lys diffusion as well as adsorption and retention within the film as a function of the film deposition conditions and post-treatment. Additionally, an effect of Lys concentration on its mobility was probed. A combination of confocal fluorescence microscopy, fluorescence recovery after photobleaching, and microfluidics was employed for this investigation. Our main finding is that adsorption of PLL and HA after protein loading induces acceleration and reduction of Lys mobility, respectively. These results suggest that a charge balance in the film to a high extent governs the protein-film interaction. We believe that control over protein mobility is a key to reach the full potential of the PLL/HA films as reservoirs for biomolecules depending on the application demand. (C) 2016 The Authors. Published by Elsevier B.V.
KW  - Layer-by-layer
KW  - Protein
KW  - Diffusion
KW  - Release
KW  - FRAP
Y1  - 2016
U6  - https://doi.org/10.1016/j.colsurfb.2016.07.055
SN  - 0927-7765
SN  - 1873-4367
VL  - 147
SP  - 343
EP  - 350
PB  - Elsevier
CY  - Amsterdam
ER  -