TY  - JOUR
A1  - Raynaud, F
A1  - Jond-Necand, C
A1  - Marcilhac, Anne
A1  - Fürst, Dieter Oswald
A1  - Benyamin, Yves
T1  - Calpain 1-gamma filamin interaction in muscle cells : a possible in situ regulation by PKC-alpha
Y1  - 2006
ER  - 
TY  - JOUR
A1  - Raynaud, Fabrice
A1  - Jond-Necand, Carole
A1  - Marcilhac, Anne
A1  - Fürst, Dieter
A1  - Benyamin, Yves
T1  - Calpain 1-gamma filamin interaction in muscle cells : a possible in situ regulation by PKC-alpha
N2  - Calpains are a family of calcium-dependant cysteine-proteases involved in cytoskeleton remodelling and muscle differentiation. In a recent study, we observed the presence of calpain I in the muscle contractile apparatus and specifically in the N1- and N2-fines. This calpain isoform was found to be involved in the degradation of muscle fibres via proteolysis of key proteins in Z-disk and costameric junctions. The goal of this study was to determine whether gamma-filamin - a specific muscle isoform of the filamin family - is a calpain, I substrate and to characterise this interaction. gamma-Filamin is a major muscle architectural protein located in the Z-fine and under the sarcolemmal membrane. This protein is a component of the chain binding the sarcolemma to the sarcomeric structure. In this study, we found that gamma-filamin formed a stable complex in vitro and in cells with calpain I in the absence of calcium stimulation. We also located the binding domains in the C-terminus of gamma-filamin with a cleavage site between serine 2626 and serine 2627 in the hinge 2 region. The catalytic (80 kDa) and regulatory (28 kDa) subunits of calpain I are both involved in high affinity binding at gamma-filamin. Moreover, we showed that phosphorylation of the filamin C- terminus domain by PKC alpha protected gamma-filamin against proteolysis by calpain I in COS cells. Stimulation of PKC activity in myotubes, prevented gamma-filamin proteolysis by calpain and resulted in an increase in myotube adhesion.
Y1  - 2006
UR  - http://www.sciencedirect.com/science/journal/13572725
U6  - https://doi.org/10.1016/j.biocel.2005.09.020
SN  - 1357-2725
ER  - 
TY  - JOUR
A1  - Raynaud, F
A1  - Jond-Necand, C
A1  - Marcilhac, Anne
A1  - Fürst, Dieter Oswald
A1  - Benyamin, Yves
T1  - Calpain 1-gamma filamin interaction in muscle cells :a possible in situ regulation by PKC-alpha
Y1  - 2006
ER  - 
TY  - JOUR
A1  - Lavee, Hanoch
A1  - Imeson, A.
A1  - Bork, Hans-Rudolf
A1  - Shoshani, M.
A1  - Kutiel, P.
A1  - Benyamin, Yves
A1  - Sarah, P.
T1  - Eco-geomorphic systems along a climatic gradient
Y1  - 1995
ER  -