TY  - JOUR
A1  - Stanislas, Thomas
A1  - Huser, Anke
A1  - Barbosa, Ines C. R.
A1  - Kiefer, Christian S.
A1  - Brackmann, Klaus
A1  - Pietra, Stefano
A1  - Gustavsson, Anna
A1  - Zourelidou, Melina
A1  - Schwechheimer, Claus
A1  - Grebe, Markus
T1  - Arabidopsis D6PK is a lipid domain-dependent mediator of root epidermal planar polarity
JF  - Nature plants
N2  - Development of diverse multicellular organisms relies on coordination of single-cell polarities within the plane of the tissue layer (planar polarity). Cell polarity often involves plasma membrane heterogeneity generated by accumulation of specific lipids and proteins into membrane subdomains. Coordinated hair positioning along Arabidopsis root epidermal cells provides a planar polarity model in plants, but knowledge about the functions of proteo-lipid domains in planar polarity signalling remains limited. Here we show that Rho-of-plant (ROP) 2 and 6, phosphatidylinositol-4-phosphate 5-kinase 3 (PIP5K3), DYNAMIN-RELATED PROTEIN (DRP) 1A and DRP2B accumulate in a sterol-enriched, polar membrane domain during root hair initiation. DRP1A, DRP2B, PIP5K3 and sterols are required for planar polarity and the AGCVIII kinase D6 PROTEIN KINASE (D6PK) is a modulator of this process. D6PK undergoes phosphatidylinositol-4,5-bisphosphate- and sterol-dependent basal-to-planar polarity switching into the polar, lipid-enriched domain just before hair formation, unravelling lipid-dependent D6PK localization during late planar polarity signalling.
Y1  - 2015
U6  - https://doi.org/10.1038/NPLANTS.2015.162
SN  - 2055-026X
SN  - 2055-0278
VL  - 1
IS  - 11
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Poxson, David J.
A1  - Karady, Michal
A1  - Gabrielsson, Roger
A1  - Alkattan, Aziz Y.
A1  - Gustavsson, Anna
A1  - Doyle, Siamsa M.
A1  - Robert, Stephanie
A1  - Ljung, Karin
A1  - Grebe, Markus
A1  - Simon, Daniel T.
A1  - Berggren, Magnus
T1  - Regulating plant physiology with organic electronics
JF  - Proceedings of the National Academy of Sciences of the United States of America
N2  - The organic electronic ion pump (OEIP) provides flow-free and accurate delivery of small signaling compounds at high spatio-temporal resolution. To date, the application of OEIPs has been limited to delivery of nonaromatic molecules to mammalian systems, particularly for neuroscience applications. However, many long-standing questions in plant biology remain unanswered due to a lack of technology that precisely delivers plant hormones, based on cyclic alkanes or aromatic structures, to regulate plant physiology. Here, we report the employment of OEIPs for the delivery of the plant hormone auxin to induce differential concentration gradients and modulate plant physiology. We fabricated OEIP devices based on a synthesized dendritic polyelectrolyte that enables electrophoretic transport of aromatic substances. Delivery of auxin to transgenic Arabidopsis thaliana seedlings in vivo was monitored in real time via dynamic fluorescent auxin-response reporters and induced physiological responses in roots. Our results provide a starting point for technologies enabling direct, rapid, and dynamic electronic interaction with the biochemical regulation systems of plants.
KW  - auxin
KW  - Arabidopsis thaliana
KW  - dendritic polymer
KW  - bioelectronics
KW  - polyelectrolyte
Y1  - 2017
U6  - https://doi.org/10.1073/pnas.1617758114
SN  - 0027-8424
VL  - 114
SP  - 4597
EP  - 4602
PB  - National Acad. of Sciences
CY  - Washington
ER  - 
TY  - JOUR
A1  - Gendre, Delphine
A1  - Baral, Anirban
A1  - Dang, Xie
A1  - Esnay, Nicolas
A1  - Boutte, Yohann
A1  - Stanislas, Thomas
A1  - Vain, Thomas
A1  - Claverol, Stephane
A1  - Gustavsson, Anna
A1  - Lin, Deshu
A1  - Grebe, Markus
A1  - Bhalerao, Rishikesh P.
T1  - Rho-of-plant activated root hair formation requires Arabidopsis YIP4a/b gene function
JF  - Development : Company of Biologists
N2  - Root hairs are protrusions from root epidermal cells with crucial roles in plant soil interactions. Although much is known about patterning, polarity and tip growth of root hairs, contributions of membrane trafficking to hair initiation remain poorly understood. Here, we demonstrate that the trans-Golgi network-localized YPT-INTERACTING PROTEIN 4a and YPT-INTERACTING PROTEIN 4b (YIP4a/b) contribute to activation and plasma membrane accumulation of Rho-of-plant (ROP) small GTPases during hair initiation, identifying YIP4a/b as central trafficking components in ROP-dependent root hair formation.
KW  - ROP
KW  - YIP
KW  - Root hair
KW  - Secretion
KW  - Trans-Golgi network
Y1  - 2019
U6  - https://doi.org/10.1242/dev.168559
SN  - 0950-1991
SN  - 1477-9129
VL  - 146
IS  - 5
PB  - The Company of Biologists
CY  - Cambridge
ER  -