TY  - JOUR
A1  - Zaitsev-Doyle, John J.
A1  - Puchert, Anke
A1  - Pfeifer, Yannik
A1  - Yan, Hao
A1  - Yorke, Briony A.
A1  - Müller-Werkmeister, Henrike
A1  - Uetrecht, Charlotte
A1  - Rehbein, Julia
A1  - Huse, Nils
A1  - Pearson, Arwen R.
A1  - Sans, Marta
T1  - Synthesis and characterisation of alpha-carboxynitrobenzyl photocaged l-aspartates for applications in time-resolved structural biology
JF  - RSC Advances
N2  - We report a new synthetic route to a series of a-carboxynitrobenzyl photocaged L-aspartates for application in time-resolved structural biology. The resulting compounds were characterised in terms of UV/Vis absorption properties, aqueous solubility and stability, and photocleavage rates (tau = ms to ms) and quantum yields (phi = 0.05 to 0.14).
Y1  - 2019
U6  - https://doi.org/10.1039/c9ra00968j
SN  - 2046-2069
VL  - 9
IS  - 15
SP  - 8695
EP  - 8699
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Schulz, Eike C.
A1  - Mehrabi, Pedram
A1  - Müller-Werkmeister, Henrike
A1  - Tellkamp, Friedjof
A1  - Jha, Ajay
A1  - Stuart, William
A1  - Persch, Elke
A1  - De Gasparo, Raoul
A1  - Diederich, François
A1  - Pai, Emil F.
A1  - Miller, R. J. Dwayne
T1  - The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
JF  - Nature methods : techniques for life scientists and chemists
N2  - We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
KW  - Biophysical chemistry
KW  - Enzymes
KW  - Molecular biophysics
KW  - X-ray crystallography
Y1  - 2018
U6  - https://doi.org/10.1038/s41592-018-0180-2
SN  - 1548-7091
SN  - 1548-7105
VL  - 15
IS  - 11
SP  - 901
EP  - 904
PB  - Nature Publishing Group (London)
CY  - London
ER  - 
TY  - JOUR
A1  - Bapolisi, Alain Murhimalika
A1  - Kielb, Patrycja
A1  - Bekir, Marek
A1  - Lehnen, Anne-Catherine
A1  - Radon, Christin
A1  - Laroque, Sophie
A1  - Wendler, Petra
A1  - Müller-Werkmeister, Henrike
A1  - Hartlieb, Matthias
T1  - Antimicrobial polymers of linear and bottlebrush architecture
BT  - Probing the membrane interaction and physicochemical properties
JF  - Macromolecular rapid communications : publishing the newsletters of the European Polymer Federation
N2  - Polymeric antimicrobial peptide mimics are a promising alternative for the future management of the daunting problems associated with antimicrobial resistance. However, the development of successful antimicrobial polymers (APs) requires careful control of factors such as amphiphilic balance, molecular weight, dispersity, sequence, and architecture. While most of the earlier developed APs focus on random linear copolymers, the development of APs with advanced architectures proves to be more potent. It is recently developed multivalent bottlebrush APs with improved antibacterial and hemocompatibility profiles, outperforming their linear counterparts. Understanding the rationale behind the outstanding biological activity of these newly developed antimicrobials is vital to further improving their performance. This work investigates the physicochemical properties governing the differences in activity between linear and bottlebrush architectures using various spectroscopic and microscopic techniques. Linear copolymers are more solvated, thermo-responsive, and possess facial amphiphilicity resulting in random aggregations when interacting with liposomes mimicking Escheria coli membranes. The bottlebrush copolymers adopt a more stable secondary conformation in aqueous solution in comparison to linear copolymers, conferring rapid and more specific binding mechanism to membranes. The advantageous physicochemical properties of the bottlebrush topology seem to be a determinant factor in the activity of these promising APs.
KW  - antimicrobial polymers
KW  - bottlebrush copolymers
KW  - liposomes
KW  - membrane
KW  - interactions
KW  - quartz crystal microbalance
Y1  - 2022
U6  - https://doi.org/10.1002/marc.202200288
SN  - 1521-3927
SN  - 1022-1336
VL  - 43
IS  - 19
PB  - Wiley-VCH
CY  - Weinheim
ER  -