TY  - JOUR
A1  - Hochrein, Lena
A1  - Mitchell, Leslie A.
A1  - Schulz, Karina
A1  - Messerschmidt, Katrin
A1  - Müller-Röber, Bernd
T1  - L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast
JF  - Nature Communications
N2  - The synthetic yeast genome constructed by the International Synthetic Yeast Sc2.0 consortium adds thousands of loxPsym recombination sites to all 16 redesigned chromosomes, allowing the shuffling of Sc2.0 chromosome parts by the Cre-loxP recombination system thereby enabling genome evolution experiments. Here, we present L-SCRaMbLE, a lightcontrolled Cre recombinase for use in the yeast Saccharomyces cerevisiae. L-SCRaMbLE allows tight regulation of recombinase activity with up to 179-fold induction upon exposure to red light. The extent of recombination depends on induction time and concentration of the chromophore phycocyanobilin (PCB), which can be easily adjusted. The tool presented here provides improved recombination control over the previously reported estradiol-dependent SCRaMbLE induction system, mediating a larger variety of possible recombination events in SCRaMbLE-ing a reporter plasmid. Thereby, L-SCRaMbLE boosts the potential for further customization and provides a facile application for use in the S. cerevisiae genome reengineering project Sc2.0 or in other recombination-based systems.
Y1  - 2018
U6  - https://doi.org/10.1038/s41467-017-02208-6
SN  - 2041-1723
VL  - 9
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - GEN
A1  - Messerschmidt, Katrin
A1  - Machens, Fabian
A1  - Hochrein, Lena
A1  - Naseri, Gita
T1  - Orthogonal, light-inducible protein expression platform in yeast Sacchararomyces cerevisiae
T2  - New biotechnology
Y1  - 2018
U6  - https://doi.org/10.1016/j.nbt.2018.05.153
SN  - 1871-6784
SN  - 1876-4347
VL  - 44
SP  - S19
EP  - S19
PB  - Elsevier
CY  - Amsterdam
ER  -