TY  - GEN
A1  - Sas, Claudia
A1  - Müller, Frank
A1  - Kappel, Christian
A1  - Kent, Tyler V.
A1  - Wright, Stephen I.
A1  - Hilker, Monika
A1  - Lenhard, Michael
T1  - Repeated inactivation of the first committed enzyme underlies the loss of benzaldehyde emission after the selfing transition in Capsella
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The enormous species richness of flowering plants is at least partly due to floral diversification driven by interactions between plants and their animal pollinators [1, 2]. Specific pollinator attraction relies on visual and olfactory floral cues [3-5]; floral scent can not only attract pollinators but also attract or repel herbivorous insects [6-8]. However, despite its central role for plant-animal interactions, the genetic control of floral scent production and its evolutionary modification remain incompletely understood [9-13]. Benzenoids are an important class of floral scent compounds that are generated from phenylalanine via several enzymatic pathways [14-17]. Here we address the genetic basis of the loss of floral scent associated with the transition from outbreeding to selfing in the genus Capsella. While the outbreeding C. grandiflora emits benzaldehyde as a major constituent of its floral scent, this has been lost in the selfing C. rubella. We identify the Capsella CNL1 gene encoding cinnamate: CoA ligase as responsible for this variation. Population genetic analysis indicates that CNL1 has been inactivated twice independently in C. rubella via different novel mutations to its coding sequence. Together with a recent study in Petunia [18], this identifies cinnamate: CoA ligase as an evolutionary hotspot for mutations causing the loss of benzenoid scent compounds in association with a shift in the reproductive strategy of Capsella from pollination by insects to self-fertilization.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 904 
KW  - benzyl alcohol-dehydrogenase
KW  - floral scent
KW  - recent speciation
KW  - petunia flowers
KW  - genus capsella
KW  - evolution
KW  - biosynthesis
KW  - fragrance
KW  - purification
KW  - pollinators
KW  - benzaldehyde
KW  - selfing syndrome
KW  - shepherd’s purse
KW  - cinnamate:CoA ligase
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-438018
SN  - 1866-8372
IS  - 904
SP  - 3313
EP  - 3319
ER  - 
TY  - GEN
A1  - Jantzen, Friederike
A1  - Lynch, Joseph H.
A1  - Kappel, Christian
A1  - Höfflin, Jona
A1  - Skaliter, Oded
A1  - Wozniak, Natalia Joanna
A1  - Sicard, Adrien
A1  - Sas, Claudia
A1  - Adebesin, Funmilayo
A1  - Ravid, Jasmin
A1  - Vainstein, Alexander
A1  - Hilker, Monika
A1  - Dudareva, Natalia
A1  - Lenhard, Michael
T1  - Retracing the molecular basis and evolutionary history of the loss of benzaldehyde emission in the genus Capsella
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - The transition from pollinator-mediated outbreeding to selfing has occurred many times in angiosperms. This is generally accompanied by a reduction in traits attracting pollinators, including reduced emission of floral scent. In Capsella, emission of benzaldehyde as a main component of floral scent has been lost in selfing C. rubella by mutation of cinnamate-CoA ligase CNL1. However, the biochemical basis and evolutionary history of this loss remain unknown, as does the reason for the absence of benzaldehyde emission in the independently derived selfer Capsella orientalis. We used plant transformation, in vitro enzyme assays, population genetics and quantitative genetics to address these questions. CNL1 has been inactivated twice independently by point mutations in C. rubella, causing a loss of enzymatic activity. Both inactive haplotypes are found within and outside of Greece, the centre of origin of C. rubella, indicating that they arose before its geographical spread. By contrast, the loss of benzaldehyde emission in C. orientalis is not due to an inactivating mutation in CNL1. CNL1 represents a hotspot for mutations that eliminate benzaldehyde emission, potentially reflecting the limited pleiotropy and large effect of its inactivation. Nevertheless, even closely related species have followed different evolutionary routes in reducing floral scent.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 775 
KW  - benzaldehyde
KW  - Capsella
KW  - cinnamate-CoA ligase
KW  - evolution
KW  - floral scent
KW  - selfing syndrome
KW  - shepherd’s purse
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-437542
SN  - 1866-8372
IS  - 775
SP  - 1349
EP  - 1360
ER  -