TY  - JOUR
A1  - Torjek, Otto
A1  - Witucka-Wall, Hanna
A1  - Meyer, Rhonda C.
A1  - von Korff, Maria
A1  - Kusterer, Barbara
A1  - Rautengarten, Carsten
A1  - Altmann, Thomas
T1  - Segregation distortion in Arabidopsis C24/Col-0 and Col-0/C24 recombinant inbred line populations is due to reduced fertility caused by epistatic interaction of two loci
JF  - Theoretical and applied genetics
N2  - A new large set of reciprocal recombinant inbred lines (RILs) was created between the Arabidopsis accessions Col-0 and C24 for quantitative trait mapping approaches, consisting of 209 Col-0 x C24 and 214 C24 x Col-0 F-7 RI lines. Genotyping was performed using 110 evenly distributed framework single nucleotide polymorphism markers, yielding a genetic map of 425.70 cM, with an average interval of 3.87 cM. Segregation distortion (SD) was observed in several genomic regions during the construction of the genetic map. Linkage disequilibrium analysis revealed an association between a distorted region at the bottom of chromosome V and a non-distorted region on chromosome IV. A detailed analysis of the RILs for these two regions showed that an SD occurred when homozygous Col-0 alleles on chromosome IV coincided with homozygous C24 alleles at the bottom of chromosome V. Using nearly isogenic lines segregating for the distorted region we confirmed that this genotypic composition leads to reduced fertility and fitness.
Y1  - 2006
U6  - https://doi.org/10.1007/s00122-006-0402-3
SN  - 0040-5752
VL  - 113
SP  - 1551
EP  - 1561
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Schmid, Karl J.
A1  - Torjek, Otto
A1  - Meyer, Rhonda C.
A1  - Schmuths, Heike
A1  - Hoffmann, Matthias H.
A1  - Altmann, Thomas
T1  - Evidence for a large-scale population structure of Arabidopsis thaliana from genome-wide single nucleotide polymorphism markers
N2  - Population-based methods for the genetic mapping of adaptive traits and the analysis of natural selection require that the population structure and demographic history of a species are taken into account. We characterized geographic patterns of genetic variation in the model plant Arabidopsis thaliana by genotyping 115 genome-wide single nucleotide polymorphism (SNP) markers in 351 accessions from the whole species range using a matrix-assisted laser desorption/ionization time-of-flight assay, and by sequencing of nine unlinked short genomic regions in a subset of 64 accessions. The observed frequency distribution of SNPs is not consistent with a constant-size neutral model of sequence polymorphism due to an excess of rare polymorphisms. There is evidence for a significant population structure as indicated by differences in genetic diversity between geographic regions. Accessions from Central Asia have a low level of polymorphism and an increased level of genome-wide linkage disequilibrium (LD) relative to accessions from the Iberian Peninsula and Central Europe. Cluster analysis with the structure program grouped Eurasian accessions into K=6 clusters. Accessions from the Iberian Peninsula and from Central Asia constitute distinct populations, whereas Central and Eastern European accessions represent admixed populations in which genomes were reshuffled by historical recombination events. These patterns likely result from a rapid postglacial recolonization of Eurasia from glacial refugial populations. Our analyses suggest that mapping populations for association or LD mapping should be chosen from regional rather than a species-wide sample or identified genetically as sets of individuals with similar average genetic distances
Y1  - 2006
UR  - http://www.springerlink.com/content/d1683r7455648841/fulltext.html
U6  - https://doi.org/10.1007/s00122-006-0212-7
ER  -