TY - JOUR A1 - Watanabe, Mutsumi A1 - Tohge, Takayuki A1 - Balazadeh, Salma A1 - Erban, Alexander A1 - Giavalisco, Patrick A1 - Kopka, Joachim A1 - Mueller-Roeber, Bernd A1 - Fernie, Alisdair A1 - Hoefgen, Rainer T1 - Comprehensive Metabolomics Studies of Plant Developmental Senescence JF - Plant Senescence: Methods and Protocols N2 - Leaf senescence is an essential developmental process that involves diverse metabolic changes associated with degradation of macromolecules allowing nutrient recycling and remobilization. In contrast to the significant progress in transcriptomic analysis of leaf senescence, metabolomics analyses have been relatively limited. A broad overview of metabolic changes during leaf senescence including the interactions between various metabolic pathways is required to gain a better understanding of the leaf senescence allowing to link transcriptomics with metabolomics and physiology. In this chapter, we describe how to obtain comprehensive metabolite profiles and how to dissect metabolic shifts during leaf senescence in the model plant Arabidopsis thaliana. Unlike nucleic acid analysis for transcriptomics, a comprehensive metabolite profile can only be achieved by combining a suite of analytic tools. Here, information is provided for measurements of the contents of chlorophyll, soluble proteins, and starch by spectrophotometric methods, ions by ion chromatography, thiols and amino acids by HPLC, primary metabolites by GC/TOF-MS, and secondary metabolites and lipophilic metabolites by LC/ESI-MS. These metabolite profiles provide a rich catalogue of metabolic changes during leaf senescence, which is a helpful database and blueprint to be correlated to future studies such as transcriptome and proteome analyses, forward and reverse genetic studies, or stress-induced senescence studies. KW - Senescence KW - Metabolomics KW - Arabidopsis KW - GC/MS KW - LC/MS KW - HPLC KW - IC Y1 - 2018 SN - 978-1-4939-7672-0 SN - 978-1-4939-7670-6 U6 - https://doi.org/10.1007/978-1-4939-7672-0_28 SN - 1064-3745 SN - 1940-6029 VL - 1744 SP - 339 EP - 358 PB - Humana Press CY - Totowa ER - TY - JOUR A1 - de Wiljes, Jana A1 - Reich, Sebastian A1 - Stannat, Wilhelm T1 - Long-Time stability and accuracy of the ensemble Kalman-Bucy Filter for fully observed processes and small measurement noise JF - SIAM Journal on Applied Dynamical Systems N2 - The ensemble Kalman filter has become a popular data assimilation technique in the geosciences. However, little is known theoretically about its long term stability and accuracy. In this paper, we investigate the behavior of an ensemble Kalman-Bucy filter applied to continuous-time filtering problems. We derive mean field limiting equations as the ensemble size goes to infinity as well as uniform-in-time accuracy and stability results for finite ensemble sizes. The later results require that the process is fully observed and that the measurement noise is small. We also demonstrate that our ensemble Kalman-Bucy filter is consistent with the classic Kalman-Bucy filter for linear systems and Gaussian processes. We finally verify our theoretical findings for the Lorenz-63 system. KW - data assimilation KW - Kalman Bucy filter KW - ensemble Kalman filter KW - stability KW - accuracy KW - asymptotic behavior Y1 - 2018 U6 - https://doi.org/10.1137/17M1119056 SN - 1536-0040 VL - 17 IS - 2 SP - 1152 EP - 1181 PB - Society for Industrial and Applied Mathematics CY - Philadelphia ER - TY - JOUR A1 - Thienen, Julia von A1 - Clancey, William J. A1 - Corazza, Giovanni Emanuele A1 - Meinel, Christoph T1 - Theoretical foundations of design thinking creative thinking theories JF - Design Thinking Research: Making Distinctions: Collaboration versus Cooperation N2 - Design thinking is acknowledged as a thriving innovation practice plus something more, something in the line of a deep understanding of innovation processes. At the same time, quite how and why design thinking works-in scientific terms-appeared an open question at first. Over recent years, empirical research has achieved great progress in illuminating the principles that make design thinking successful. Lately, the community began to explore an additional approach. Rather than setting up novel studies, investigations into the history of design thinking hold the promise of adding systematically to our comprehension of basic principles. This chapter makes a start in revisiting design thinking history with the aim of explicating scientific understandings that inform design thinking practices today. It offers a summary of creative thinking theories that were brought to Stanford Engineering in the 1950s by John E. Arnold. Y1 - 2018 SN - 978-3-319-60967-6 SN - 978-3-319-60966-9 U6 - https://doi.org/10.1007/978-3-319-60967-6_2 SP - 13 EP - 40 PB - Springer CY - New York ER - TY - JOUR A1 - Schmidt, Silke Regina A1 - Gerten, Dieter A1 - Hintze, Thomas A1 - Lischeid, Gunnar A1 - Livingstone, David M. A1 - Adrian, Rita T1 - Temporal and spatial scales of water temperature variability as an indicator for mixing in a polymictic lake JF - Inland waters : journal of the International Society of Limnology N2 - We applied coarse spectral analysis to more than 2 decades of daily near-surface water temperature (WT) measurements from Muggelsee, a shallow polymictic lake in Germany, to systematically characterize patterns in WT variability from daily to yearly temporal scales. Comparison of WT with local air temperature indicates that the WT variability patterns are likely attributable to both meteorological forcing and internal lake dynamics. We identified seasonal patterns of WT variability and showed that WT variability increases with increasing Schmidt stability, decreasing Lake number and decreasing ice cover duration, and is higher near the shore than in open water. We introduced the slope of WT spectra as an indicator for the degree of lake mixing to help explain the identified temporal and spatial scales of WT variability. The explanatory power of this indicator in other lakes with different mixing regimes remains to be established. KW - Lake number KW - polymictic lakes KW - Schmidt stability KW - seasonality KW - spectral analysis KW - variability Y1 - 2018 U6 - https://doi.org/10.1080/20442041.2018.1429067 SN - 2044-2041 SN - 2044-205X VL - 8 IS - 1 SP - 82 EP - 95 PB - Routledge, Taylor & Francis Group CY - Abingdon ER - TY - JOUR A1 - Krüger-Genge, Anne A1 - Schulz, Christian A1 - Kratz, Karl A1 - Lendlein, Andreas A1 - Jung, Friedrich T1 - Comparison of two substrate materials used as negative control in endothelialization studies BT - Glass versus polymeric tissue culture plate JF - Clinical hemorheology and microcirculation : blood flow and vessels N2 - The endothelialization of synthetic surfaces applied as cardiovascular implant materials is an important issue to ensure the anti-thrombotic quality of a biomaterial. However, the rapid and constant development of a functionallycon-fluent endothelial cell monolayer is challenging. In order to investigate the compatibility of potential implant materials with endothelial cells several in vitro studies are performed. Here, glass and tissue culture plates (TCP) are often used as reference materials for in vitro pre-testing. However, a direct comparison of both substrates is lacking. Therefore, a comparison of study results is difficult, since results are often related to various reference materials. In this study, the endothelialization of glass and TCP was investigated in terms of adherence, morphology, integrity, viability and function using human umbilical vein endothelial cells (HUVEC). On both substrates an almost functionally confluent HUVEC monolayer was developed after nine days of cell seeding with clearly visible cell rims, decreased stress fiber formation and a pronounced marginal filament band. The viability of HUVEC was comparable for both substrates nine days after cell seeding with only a few dead cells. According to that, the cell membrane integrity as well as the metabolic activity showed no differences between TCP and glass. However, a significant difference was observed for the secretion of IL-6 and IL-8. The concentration of both cytokines, which are associated with migratory activity, was increased in the supernatant of HUVEC seeded on TCP. This result matches well with the slightly increased number of adherent HUVEC on TCP. In conclusion, these findings indicate that both reference materials are almost comparable and can be used equivalently as control materials in in vitro endothelialization studies. KW - Negative control KW - endothelial cells KW - glass KW - TCP KW - reference Y1 - 2018 U6 - https://doi.org/10.3233/CH-189904 SN - 1386-0291 SN - 1875-8622 VL - 69 IS - 3 SP - 437 EP - 445 PB - IOS Press CY - Amsterdam ER - TY - JOUR A1 - Krüger-Genge, A. A1 - Braune, S. A1 - Walter, M. A1 - Krengel, M. A1 - Kratz, K. A1 - Küpper, J. H. A1 - Lendlein, Andreas A1 - Jung, Friedrich T1 - Influence of different surface treatments of poly(n-butyl acrylate) networks on fibroblasts adhesion, morphology and viability JF - Clinical hemorheology and microcirculation : blood flow and vessels N2 - BACKGROUND: Physical and chemical characteristics of implant materials determine the fate of long-term cardiovascular devices. However, there is still a lack of fundamental understanding of the molecular mechanisms occurring in the material-tissue interphase. In a previous study, soft covalently crosslinked poly(n-butyl acrylate) networks (cPnBA) were introduced as sterilizable, non-toxic and immuno-compatible biomaterials with mechanical properties adjustable to blood vessels. Here we study the influence of different surface treatments in particular oxygen plasma modification and fibrinogen deposition as well as a combinatorial approach on the adhesion and viability of fibroblasts. RESULTS: Compared to non-treated cPnBAs the advancing water-contact angles were found to be reduced after all surface modifications (p<0.05, each), while lowest values were observed after the combined surface treatment (OPT+FIB). The latter differed significantly from the single OPT and FIB. The number of adherent fibroblasts and their adherence behavior differed on both pristine cPnBA networks. The fibroblast density on cPnBA04 was 743 +/- 434 cells. mm(-2), was about 6.5 times higher than on cPnBA73 with 115 +/- 73 cells. mm(-2). On cPnBA04 about 20% of the cells were visible as very small, round and buckled cells while all other cells were in a migrating status. On cPnBA73, nearly 50% of fibroblasts were visible as very small, round and buckled cells. The surface functionalization either using oxygen plasma treatment or fibrinogen coating led to a significant increase of adherent fibroblasts, particularly the combination of both techniques, for both cPnBA networks. It is noteworthy to mention that the fibrinogen coating overruled the characteristics of the pristine surfaces; here, the fibroblast densities after seeding were identical for both cPnBAnetworks. Thus, the binding rather depended on the fibrinogen coating than on the substrate characteristics anymore. While the integrity of the fibroblasts membrane was comparable for both polymers, the MTS tests showed a decreased metabolic activity of the fibroblasts on cPnBA. CONCLUSION: The applied surface treatments of cPnBA successfully improved the adhesion of viable fibroblasts. Under resting conditions as well as after shearing the highest fibroblast densities were found on surfaces with combined post-treatment. KW - Biomaterial KW - poly(n-butyl acrylate) KW - fibroblast KW - oxygen plasma KW - fibrinogen KW - cell adhesion KW - focal adhesion KW - actin cytoskeleton KW - viability Y1 - 2018 U6 - https://doi.org/10.3233/CH-189130 SN - 1386-0291 SN - 1875-8622 VL - 69 IS - 1-2 SP - 305 EP - 316 PB - IOS Press CY - Amsterdam ER - TY - JOUR A1 - Kumar, Reddi K. A1 - Heuchel, Matthias A1 - Kratz, Karl A1 - Lendlein, Andreas A1 - Jankowski, Joachim A1 - Tetali, Sarada D. T1 - Effects of extracts prepared from modified porous poly(ether imide) microparticulate absorbers on cytotoxicity, macrophage differentiation and proinflammatory behavior of human monocytic (THP-1) cells JF - Clinical hemorheology and microcirculation : blood flow and vessels N2 - Remaining uremic toxins in the blood of chronic renal failure patients represent one central challenge in hemodialysis therapies. Highly porous poly(ether imide) (PEI) microparticles have been recently introduced as candidate absorber materials, which show a high absorption capacity for uremic toxins and allow hydrophilic surface modification suitable for minimization of serum protein absorption. In this work, the effects of extracts prepared from PEI microparticles modified by nucleophilic reaction with low molecular weight polyethylene imine (Pei) or potassium hydroxide (KOH), on human monocytic (THP-1) cells are studied. The obtained results suggested that the extracts of Pei and KOH modified PEI absorbers have no negative effect on THP-1 cell viability and do not initiate the critical differentiation towards macrophages. The extracts did not enhance transcript or protein levels of investigated proinflammatory markers in THP-1 cells, namely, TNF alpha, MCP1, IL6 and IL8. Based on these findings such modified PEI microparticles should be qualified for further pre-clinical evaluation i.e. in an in vivo animal experiment. KW - Chronic kidney disease KW - hemodialysis KW - Inflammation KW - Porous poly(ether imide) microparticulate absorbers KW - THP-1 cells KW - Uremic toxins Y1 - 2018 U6 - https://doi.org/10.3233/CH-189112 SN - 1386-0291 SN - 1875-8622 VL - 69 IS - 1-2 SP - 175 EP - 185 PB - IOS Press CY - Amsterdam ER - TY - JOUR A1 - Bourne, D. P. A1 - Cushing, D. A1 - Liu, S. A1 - Münch, Florentin A1 - Peyerimhoff, Norbert T1 - Ollivier-Ricci idleness functions of graphs JF - SIAM Journal on Discrete Mathematics N2 - We study the Ollivier-Ricci curvature of graphs as a function of the chosen idleness. We show that this idleness function is concave and piecewise linear with at most three linear parts, and at most two linear parts in the case of a regular graph. We then apply our result to show that the idleness function of the Cartesian product of two regular graphs is completely determined by the idleness functions of the factors. KW - Ollivier-Ricci KW - idleness KW - optimal transport Y1 - 2018 U6 - https://doi.org/10.1137/17M1134469 SN - 0895-4801 SN - 1095-7146 VL - 32 IS - 2 SP - 1408 EP - 1424 PB - Society for Industrial and Applied Mathematics CY - Philadelphia ER - TY - JOUR A1 - Soto, Paulina T1 - Una reeducación crítica: emergencia de revistas literarias digitales chilenas en el transcurso de la primera década del siglo XXI T1 - A criticism re-education: the emergence of ChileanWeb-supported literary journals in the course of the first decade of the twenty-first century JF - Estudios Avanzados N2 - Para inicios del siglo XXI se instalan nuevos medios de crítica literaria a través de soportes digitales. Estas publicaciones tensionan un campo letrado chileno que enfrenta cuestionamientos. Gestionadas mayoritariamente por creadores precedentes de la generación del 90, producen reaproximaciones a la cultura letrada. El objetivo es desafiar dinámicas de interacción lectora heredadas vinculadas a las luces patria, desarrollistas, de tipo linear y ciegas a la heterogeneidad cultural vivida en la época. El proceso comprende, de tal modo, una reeducación crítica centrada en ensayar poéticas de productividad analítico-literarias heteróclitas. Se trata de una interacción lectora diversa, sujeta a retóricas íntimas de apreciación estética, que ponen en cuestión las nociones tradicionales mayoritariamente instructiva de la cultura chilena. N2 - In the course of the first decade of the twenty-first century, Web-supported Literary Journals emerged as a mean to socialize and democratize Chilean Literary Culture. The tendency, conducted by creators associated to the 90’ generation poetry challenged traditional ways of cultural interaction as well as encouraged aesthetic communication, and otherness understanding. In this way, the tendency encouraged a cultural scenario in dispute. Traditional cultural institutions appeared to be corrupted by the post dictatorial progressivist national ethos. Linked to an affective and dialogic reader-encounter, the new critical approach shifted the traditional notion of lectureship as national instruction. KW - web-supported literary journals KW - literary canon KW - chilean literary critic KW - literature and education KW - Isabel Allende Y1 - 2018 SN - 0718-5022 SN - 0718-5014 IS - 28 SP - 121 EP - 137 PB - Universidad de Santiago de Chile (USACH) CY - Santiago ER - TY - JOUR A1 - Martinez-Chicharro, M. A1 - Torrejon, J. M. A1 - Oskinova, Lidia M. A1 - Furst, F. A1 - Postnov, K. A1 - Rodes-Roca, J. J. A1 - Hainich, Rainer A1 - Bodaghee, A. T1 - Evidence of Compton cooling during an X-ray flare supports a neutron star nature of the compact object in 4U1700-37 JF - Monthly notices of the Royal Astronomical Society N2 - Based on new Chandra X-ray telescope data, we present empirical evidence of plasma Compton cooling during a flare in the non-pulsating massive X-ray binary 4U1700-37. This behaviour might be explained by quasi-spherical accretion on to a slowly rotating magnetized neutron star (NS). In quiescence, the NS in 4U1700-37 is surrounded by a hot radiatively cooling shell. Its presence is supported by the detection of mHz quasi-periodic oscillations likely produced by its convection cells. The high plasma temperature and the relatively low X-ray luminosity observed during the quiescence, point to a small emitting area similar to 1 km, compatible with a hotspot on an NS surface. The sudden transition from a radiative to a significantly more efficient Compton cooling regime triggers an episode of enhanced accretion resulting in a flare. During the flare, the plasma temperature drops quickly. The predicted luminosity for such transitions, similar to 3 x 10(35) erg s(-1), is very close to the luminosity of 4U1700-37 during quiescence. The transition may be caused by the accretion of a clump in the stellar wind of the donor star. Thus, a magnetized NS nature of the compact object is strongly favoured. KW - stars: individual: 4U1700-37 KW - V*V884 Sco KW - X-rays: binaries Y1 - 2017 U6 - https://doi.org/10.1093/mnrasl/slx165 SN - 0035-8711 SN - 1365-2966 VL - 473 IS - 1 SP - L74 EP - L78 PB - Oxford Univ. Press CY - Oxford ER -