TY - JOUR A1 - Badalyan, Artavazd A1 - Dierich, Marlen A1 - Stiba, Konstanze A1 - Schwuchow, Viola A1 - Leimkühler, Silke A1 - Wollenberger, Ulla T1 - Electrical wiring of the aldehyde oxidoreductase PaoABC with a polymer containing osmium redox centers BT - biosensors for benzaldehyde and GABA JF - Biosensors N2 - Biosensors for the detection of benzaldehyde and g-aminobutyric acid (GABA) are reported using aldehyde oxidoreductase PaoABC from Escherichia coli immobilized in a polymer containing bound low potential osmium redox complexes. The electrically connected enzyme already electrooxidizes benzaldehyde at potentials below −0.15 V (vs. Ag|AgCl, 1 M KCl). The pH-dependence of benzaldehyde oxidation can be strongly influenced by the ionic strength. The effect is similar with the soluble osmium redox complex and therefore indicates a clear electrostatic effect on the bioelectrocatalytic efficiency of PaoABC in the osmium containing redox polymer. At lower ionic strength, the pH-optimum is high and can be switched to low pH-values at high ionic strength. This offers biosensing at high and low pH-values. A “reagentless” biosensor has been formed with enzyme wired onto a screen-printed electrode in a flow cell device. The response time to addition of benzaldehyde is 30 s, and the measuring range is between 10–150 µM and the detection limit of 5 µM (signal to noise ratio 3:1) of benzaldehyde. The relative standard deviation in a series (n = 13) for 200 µM benzaldehyde is 1.9%. For the biosensor, a response to succinic semialdehyde was also identified. Based on this response and the ability to work at high pH a biosensor for GABA is proposed by coimmobilizing GABA-aminotransferase (GABA-T) and PaoABC in the osmium containing redox polymer. KW - redox polymer KW - aldehyde oxidoreductase KW - ionic strength KW - benzaldehyde KW - GABA KW - biosensor Y1 - 2014 U6 - https://doi.org/10.3390/bios4040403 VL - 4 IS - 4 SP - 403 EP - 421 PB - MDPI CY - Basel ER - TY - JOUR A1 - Küttner, Uwe-Alexander T1 - Rhythmic analyses as a proof-procedure? BT - An initial observation on rhythmicity and projection JF - Prosodie und Phonetik in der Interaktion = Prosody and phonetics in interaction N2 - This paper reports a problematic case of unequivocally evidencing participant orientation to the projective force of some turn-initial demonstrative wh-clefts (DCs) within the framework of Conversation Analysis (CA) and Interactional Linguistics (IL). Conducting rhythmic analyses appears helpful in this regard, in that they disclose rhythmic regularities which suggest a speaker's orientation towards a projected turn continuation. In this particular case, rhythmic analyses can therefore be shown to meaningfully complement sequential analyses and analyses of turn-design, so as to gather additional evidence for participant orientations. In conclusion, I will point to possibly more extensive relations between rhythmicity and projection and proffer a tentative outlook for the usability of rhythmic analyses as an analytic tool in CA and IL. N2 - Dieser primär interaktional-linguistisch ausgerichtete Beitrag berichtet von einem Problemfall im Nachweis von Teilnehmerorientierung an den Turn-Fortführung projizierenden Eigenschaften einiger turn-initialer "Demonstrative-Cleft- Konstruktionen" (DCs). Es wird gezeigt, dass rhythmische Analysen diesbezüglich hilfreich sein können, da sie im konkreten Fall rhythmische Regularitäten erkennen lassen, die eine sprecherseitige Orientierung an einer projizierten Turn-Fortführung nahelegen. Vorausgegangene sequenzielle Analysen und Analysen von Turn-Design werden somit bedeutsam durch rhythmische Analysen ergänzt, um zusätzliche Evidenzen für Teilnehmerorientierungen zu erfassen. Abschließend werde ich auf potenziell weitreichendere Zusammenhänge zwischen Rhythmizität und Projektion in der Interaktion hinweisen und einen provisorischen Ausblick hinsichtlich der möglichen Verwendung rhythmischer Analysen als Analysewerkzeug in der Konversationsanalyse und der Interaktionalen Linguistik geben. KW - Interaktionale Linguistik KW - Sprechrhythmus und Rhythmische Analyse KW - Methode KW - Teilnehmerorientierung KW - Turnkonstruktionseinheiten KW - Projektion KW - Demonstrative Clefts KW - Interactional Linguistics KW - Speech Rhythm and Rhythmic Analysis KW - Method KW - Participant Orientation KW - Turn-Constructional Units KW - Projection KW - Demonstrative Clefts Y1 - 2014 UR - http://verlag-gespraechsforschung.de/2014/pdf/kuettner-audio.pdf SN - 978-3-936656-60-2 SP - 46 EP - 69 PB - Verlag für Gesprächsforschung CY - Mannheim ER - TY - JOUR A1 - Ifenthaler, Dirk T1 - Challenges of a Holistic Learning Analytics Project JF - E-Learning Symposium 2014 : Mobil und vernetzt – studieren im digitalen Zeitalter ; Potsdam, 14. November 2014 N2 - Recently, interest in collecting and mining large sets of educational data on student background and performance to conduct research on learning and instruction has developed as an area generally referred to as learning analytics. Higher education leaders are recognising the value of learning analytics for improving not only learning and teaching but also the entire educational arena. However, theoretical concepts and empirical evidence need to be generated within the fast evolving field of learning analytics. In this paper, we introduce a holistic learning analytics framework. Based on this framework, student, learning, and curriculum profiles have been developed which include relevant static and dynamic parameters for facilitating the learning analytics framework. Based on the theoretical model, an empirical study was conducted to empirically validate the parameters included in the student profile. The paper concludes with practical implications and issues for future research. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-442303 SP - 15 EP - 28 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Sprinz, Detlef F. T1 - Long-term policy problems: definition, origins, and redponses JF - Predicting the Future in Science, Economics, and Politics Y1 - 2014 SN - 978-1-78347-186-7 SP - 126 EP - 143 PB - Elgar CY - Northampton ER - TY - JOUR A1 - Cramer, Sandra A1 - Tacke, Sebastian A1 - Bornhorst, Julia A1 - Klingauf, Jürgen A1 - Schwerdtle, Tanja A1 - Galla, Hans-Joachim T1 - The Influence of Silver Nanoparticles on the Blood-Brain and the Blood-Cerebrospinal Fluid Barrier in vitro JF - Journal of Nanomedicine & Nanotechnology N2 - The use of silver nanoparticles in medical and consumer products such as wound dressings, clothing and cosmetic has increased significantly in recent years. Still, the influence of these particles on our health and especially on our brain, has not been examined adequately up to now. We studied the influence of AgEO- (Ethylene Oxide) and AgCitrate-Nanoparticles (NPs) on the protective barriers of the brain, namely the blood-brain barrier (BBB) and the blood-cerebrospinal fluid (blood-CSF) barrier in vitro. The NPs toxicity was evaluated by examining changes in membrane integrity, cell morphology, barrier properties, oxidative stress and inflammatory reactions. AgNPs decreased cell viability, disturbed barrier integrity and tight junctions and triggered oxidative stress and DNA strand breaks. However, all mentioned effects were, at least partly, suppressed by a Citrate-coating and were most pronounced in the cells of the BBB as compared to the epithelial cells representing the blood-CSF barrier. AgEO- but not AgCitrate-NPs also triggered an inflammatory reaction in porcine brain capillary endothelial cells (PBCEC), which represent the BBB. Our data indicate that AgNPs may cause adverse effects within the barriers of the brain, but their toxicity can be reduced by choosing an appropriate coating material. Y1 - 2014 U6 - https://doi.org/10.4172/2157-7439.1000225 SN - 2157-7439 VL - 5 IS - 5 ER - TY - JOUR A1 - Noble, Andrew T1 - Ultraschall Berlin BT - Festival für neue Musik, 2014 JF - Tempo: a quarterly review of modern music Y1 - 2014 U6 - https://doi.org/10.1017/S004029821400014X SN - 1478-2286 VL - 68 IS - 269 SP - 76 EP - 78 PB - Cambidge University Press CY - Cambridge ER - TY - JOUR A1 - Hafer, Jörg A1 - Kiy, Alexander A1 - Lucke, Ulrike T1 - Moodle & Co. auf dem Weg zur Personal Learning Environment JF - eleed N2 - Ausgehend von der typischen IT‐Infrastruktur für E‐Learning an Hochschulen auf der einen Seite sowie vom bisherigen Stand der Forschung zu Personal Learning Environments (PLEs) auf der anderen Seite zeigt dieser Beitrag auf, wie bestehende Werkzeuge bzw. Dienste zusammengeführt und für die Anforderungen der modernen, rechnergestützten Präsenzlehre aufbereitet werden können. Für diesen interdisziplinären Entwicklungsprozess bieten sowohl klassische Softwareentwicklungsverfahren als auch bestehende PLE‐Modelle wenig Hilfestellung an. Der Beitrag beschreibt die in einem campusweiten Projekt an der Universität Potsdam verfolgten Ansätze und die damit erzielten Ergebnisse. Dafür werden zunächst typische Lehr‐/Lern‐bzw. Kommunikations‐Szenarien identifiziert, aus denen Anforderungen an eine unterstützende Plattform abgeleitet werden. Dies führt zu einer umfassenden Sammlung zu berücksichtigender Dienste und deren Funktionen, die gemäß den Spezifika ihrer Nutzung in ein Gesamtsystem zu integrieren sind. Auf dieser Basis werden grundsätzliche Integrationsansätze und technische Details dieses Mash‐Ups in einer Gesamtschau aller relevanten Dienste betrachtet und in eine integrierende Systemarchitektur überführt. Deren konkrete Realisierung mit Hilfe der Portal‐Technologie Liferay wird dargestellt, wobei die eingangs definierten Szenarien aufgegriffen und exemplarisch vorgestellt werden. Ergänzende Anpassungen im Sinne einer personalisierbaren bzw. adaptiven Lern‐(und Arbeits‐)Umgebung werden ebenfalls unterstützt und kurz aufgezeigt. Y1 - 2014 UR - https://eleed.campussource.de/archive/10/4085 SN - 1860-7470 VL - 2014 IS - 10 ER - TY - JOUR A1 - Günther, Oliver T1 - Big Data: What is it? And what does it mean for science, economy and society? JF - Informatik-Spektrum Y1 - 2014 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84897396415&partnerID=MN8TOARS U6 - https://doi.org/10.1007/s00287-014-0783-7 SN - 0170-6012 VL - 37 IS - 2 SP - 85 EP - 86 PB - Springer Verlag CY - Heidelberg ER - TY - JOUR A1 - Wiemann, Dirk ED - Lange, Bernd-Peter ED - Bartels, Anke T1 - A Little Piece of the Shire BT - Some Versions of the Hobbit Garden JF - Hard times : deutsch-englische Zeitschrift Y1 - 2014 VL - 2014 IS - 95 SP - 24 EP - 27 ER - TY - JOUR A1 - Heyer, Vera A1 - Clahsen, Harald T1 - Late bilinguals see a scan in scanner AND in scandal: dissecting formal overlap from morphological priming in the processing of derived words JF - Bilingualism : language and cognition. N2 - Masked priming research with late (non-native) bilinguals has reported facilitation effects following morphologically derived prime words (scanner - scan). However, unlike for native speakers, there are suggestions that purely orthographic prime-target overlap (scandal - scan) also produces priming in non-native visual word recognition. Our study directly compares orthographically related and derived prime-target pairs. While native readers showed morphological but not formal overlap priming, the two prime types yielded the same magnitudes of facilitation for non-natives. We argue that early word recognition processes in a non-native language are more influenced by surface-form properties than in one's native language. KW - Masked priming KW - late bilinguals KW - derivation KW - orthographic overlap Y1 - 2014 U6 - https://doi.org/10.1017/S1366728914000662 SN - 1366-7289 SN - 1469-1841 VL - 18 IS - 3 SP - 543 EP - 550 PB - Cambridge Univ. Press CY - New York ER -