TY  - JOUR
A1  - Jannasch, Franziska
A1  - Nickel, Daniela V.
A1  - Bergmann, Manuela M.
A1  - Schulze, Matthias Bernd
T1  - A new evidence-based diet score to capture associations of food consumption and chronic disease risk
JF  - Nutrients / Molecular Diversity Preservation International (MDPI)
N2  - Previously, the attempt to compile German dietary guidelines into a diet score was predominantly not successful with regards to preventing chronic diseases in the EPIC-Potsdam study. Current guidelines were supplemented by the latest evidence from systematic reviews and expert papers published between 2010 and 2020 on the prevention potential of food groups on chronic diseases such as type 2 diabetes, cardiovascular diseases and cancer. A diet score was developed by scoring the food groups according to a recommended low, moderate or high intake. The relative validity and reliability of the diet score, assessed by a food frequency questionnaire, was investigated. The consideration of current evidence resulted in 10 key food groups being preventive of the chronic diseases of interest. They served as components in the diet score and were scored from 0 to 1 point, depending on their recommended intake, resulting in a maximum of 10 points. Both the reliability (r = 0.53) and relative validity (r = 0.43) were deemed sufficient to consider the diet score as a stable construct in future investigations. This new diet score can be a promising tool to investigate dietary intake in etiological research by concentrating on 10 key dietary determinants with evidence-based prevention potential for chronic diseases.
KW  - diet score
KW  - dietary guidelines
KW  - food groups
KW  - chronic disease
KW  - type 2
KW  - diabetes
KW  - cardiovascular disease
KW  - cancer
KW  - prevention
KW  - reliability;
KW  - validity
Y1  - 2022
U6  - https://doi.org/10.3390/nu14112359
SN  - 2072-6643
VL  - 14
IS  - 11
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Müller, Sandra
A1  - Dawczynski, Christine
A1  - Wiest, Johanna
A1  - Lorkowski, Stefan
A1  - Kipp, Anna Patricia
A1  - Schwerdtle, Tanja
T1  - Functional Biomarkers for the Selenium Status in a Human Nutritional Intervention Study
JF  - Nutrients
N2  - Soils in Germany are commonly low in selenium; consequently, a sufficient dietary supply is not always ensured. The extent of such provision adequacy is estimated by the optimal effect range of biomarkers, which often reflects the physiological requirement. Preceding epidemiological studies indicate that low selenium serum concentrations could be related to cardiovascular diseases. Inter alia, risk factors for cardiovascular diseases are physical inactivity, overweight, as well as disadvantageous eating habits. In order to assess whether these risk factors can be modulated, a cardio-protective diet comprising fixed menu plans combined with physical exercise was applied in the German MoKaRi (modulation of cardiovascular risk factors) intervention study. We analyzed serum samples of the MoKaRi cohort (51 participants) for total selenium, GPx activity, and selenoprotein P at different timepoints of the study (0, 10, 20, 40 weeks) to explore the suitability of these selenium-associated markers as indicators of selenium status. Overall, the time-dependent fluctuations in serum selenium concentration suggest a successful change in nutritional and lifestyle behavior. Compared to baseline, a pronounced increase in GPx activity and selenoprotein P was observed, while serum selenium decreased in participants with initially adequate serum selenium content. SELENOP concentration showed a moderate positive monotonic correlation (r = 0.467, p < 0.0001) to total Se concentration, while only a weak linear relationship was observed for GPx activity versus total Se concentration (r = 0.186, p = 0.021). Evidently, other factors apart from the available Se pool must have an impact on the GPx activity, leading to the conclusion that, without having identified these factors, GPx activity should not be used as a status marker for Se
KW  - Se
KW  - selenoprotein P
KW  - GPx activity
KW  - cardiovascular disease
KW  - status markers
Y1  - 2020
U6  - https://doi.org/10.3390/nu12030676
SN  - 2072-6643
VL  - 12
IS  - 3
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Chaykovska, Lyubov
A1  - Zientara, Alicja
A1  - Reser, Diana
A1  - Weise, Alexander
A1  - Reichert, Wolfgang
A1  - Hocher, Berthold
T1  - Development and validation of a macroarray system - MutaCHIP (R) ARTERO - for the detection of genetic variants involved in the pathogenesis of atherosclerosis
JF  - Clinical laboratory : the peer reviewed journal for clinical laboratories and laboratories related to blood transfusion
N2  - Background: Cardiovascular diseases are the leading cause of death in developed countries. The underlying mechanism is often atherosclerotic remodeling of blood vessels in organs such as heart, kidney, brain, and large arteries in case of peripheral arterial disease. Beside environmental and behavioral factors such as smoking or lack of physical activity, genetic variants in genes involved in lipid metabolism, blood pressure regulation, oxidative stress, and coagulation play a prominent role in the pathogenesis of atherosclerosis.
 Methods: Thus, we developed and validated for clinical use and research a macroarray system for the simultaneous detection of key genetic variants in genes involved in lipid metabolism, blood pressure regulation, oxidative stress, and coagulation.
 Results: When compared with standard PCR technologies to determine all these genetic variants in parallel, the macroarray system (MutaCHIP (R) ARTERO) was as accurate but faster, cheaper, and easier to handle compared to classical real time PCR based technologies.
 Conclusions: MutaCHIP (R) ARTERO is a gene chip for diagnostics of a complex genetic panel involved in the pathogenesis of atherosclerosis. This method is as sensitive and precise as real time PCR and is able to replicate real time PCR data previously validated in evaluation studies.
KW  - cardiovascular disease
KW  - atherosclerosis
KW  - genes
KW  - rapid detection method
KW  - macroarray
Y1  - 2014
U6  - https://doi.org/10.7754/Clin.Lab.2014.140104
SN  - 1433-6510
VL  - 60
IS  - 5
SP  - 873
EP  - 878
PB  - Clin Lab Publ., Verl. Klinisches Labor
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Chen, You-Peng
A1  - Lu, Yong-Ping
A1  - Li, Jian
A1  - Liu, Zhi-Wei
A1  - Chen, Wen-Jing
A1  - Liang, Xu-Jing
A1  - Chen, Xin
A1  - Wen, Wang-Rong
A1  - Xiao, Xiao-Min
A1  - Reichetzeder, Christoph
A1  - Hocher, Berthold
T1  - Fetal and maternal angiotensin (1-7) are associated with preterm birth
JF  - Journal of hypertension
N2  - Background: Recent studies show that preterm birth is associated with hypertension in later life. The renin-angiotensin system (RAS) during pregnancy influences fetal growth and development. In the current study, we investigated the impact of fetal as well as maternal angiotensin (1-7) [Ang (1-7)] and angiotensin II (Ang II) plasma concentrations on the risk of preterm birth.
 Methods: Three hundred and nine pregnant women were prospectively included into the study. The pregnant women were divided into two groups, for example, preterm birth of lower than 37 gestational weeks (n = 17) and full-term birth of 37 gestational weeks or more (n = 292). Maternal and neonatal plasma Ang (1-7) and Ang II concentrations were analyzed at birth from maternal venous blood and umbilical cord blood, respectively. Risk factors for premature birth were determined by multiple logistic regression analysis.
 Results: Fetal and maternal plasma Ang (1-7) concentrations in the preterm group were lower than those of the term group fetal Ang (1-7) preterm birth: 486.15 +/- 337.34 ng/l and fetal Ang (1-7) term birth: 833.84 +/- 698.12 ng/l and maternal Ang (1-7) preterm birth: 399.86 +/- 218.93 ng/l; maternal Ang (1-7) term birth: 710.34 +/- 598.22 ng/l. Multiple logistic regression analysis considering confounding factors revealed that preeclampsia (P < 0.001), premature rupture of membranes (P = 0.001), lower concentration of maternal Ang (1-7) (P = 0.013) and fetal plasma Ang (1-7) (P = 0.032) were independently associated with preterm birth. We could furthermore demonstrate that the maternal Ang (1-7)/Ang II ratio is independently associated with gestational hypertension or preeclampsia, factors causing preterm birth.
 Conclusions: Lower concentrations of maternal and fetal Ang (1-7) are independently associated with preterm birth - a risk factor of hypertension in later life.
KW  - angiotensin (1-7)
KW  - angiotensin II
KW  - cardiovascular disease
KW  - fetal programming
KW  - intrauterine fetal growth
KW  - pregnancy
KW  - preterm delivery
Y1  - 2014
U6  - https://doi.org/10.1097/HJH.0000000000000251
SN  - 0263-6352
SN  - 1473-5598
VL  - 32
IS  - 9
SP  - 1833
EP  - 1841
PB  - Lippincott Williams & Wilkins
CY  - Philadelphia
ER  -