TY - JOUR A1 - Altmann, Thomas A1 - Udvardi, M. K. A1 - Essigmann, B. A1 - Colebatch, G. A1 - Kloska, Sebastian A1 - Smith, P. A1 - Trevaskis, B. T1 - Lotus japonicus functional genomics : cDNA microarray analysis uncovers novel nodulins Y1 - 2002 SN - 0-85199-591-8 ER - TY - JOUR A1 - Altmann, Thomas A1 - Colebatch, G. A1 - Kloska, Sebastian A1 - Trevaskis, B. A1 - Freund, S. A1 - Udvardi, M. K. T1 - Novel aspects of symbiotic nitrogen fixation uncovered by transcript profiling with cDNA arrays Y1 - 2002 ER - TY - JOUR A1 - Wood, C. C. A1 - Poree, Fabien A1 - Dreyer, Ingo A1 - Koehler, G. J. A1 - Udvardi, M. K. T1 - Mechanisms of ammonium transport, accumulation, and retention in ooyctes and yeast cells expressing Arabidopsis AtAMT1; 1 N2 - Ammonium is a primary source of N for plants, so knowing how it is transported, stored, and assimilated in plant cells is important for rational approaches to optimise N-use in agriculture. Electrophysiological studies of Arabidopsis AtAMT1;1 expressed in oocytes revealed passive, Delta psi-driven transport of NH4+ through this protein. Expression of AtAMT1;1 in a novel yeast mutant defective in endogenous ammonium transport and vacuolar acidification supported the above mechanism for AtAMT1;1 and revealed a central role for acid vacuoles in storage and retention of ammonia in cells. These results highlight the mechanistic differences between plant AMT proteins and related transporters in bacteria and animal cells, and suggest novel strategies to enhance nitrogen use efficiency in agriculture. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved Y1 - 2006 UR - http://www.sciencedirect.com/science/article/pii/S0014579306007332 U6 - https://doi.org/10.1016/j.febslet.2006.06.026 ER -