TY  - JOUR
A1  - Hüttl, Christine
A1  - Hettrich, Cornelia
A1  - Riedel, Melanie
A1  - Henklein, Petra
A1  - Rawel, Harshadrai Manilal
A1  - Bier, Frank Fabian
T1  - Development of Peptidyl Lysine Dendrons: 1,3-Dipolar Cycloaddition for Peptide Coupling and Antibody Recognition
JF  - Chemical biology & drug design
N2  - A straightforward synthesis strategy to multimerize a peptide mimotopes for antibody B13-DE1 recognition is described based on lysine dendrons as multivalent scaffolds. Lysine dendrons that possess N-terminal alkyne residues at the periphery were quantitative functionalized with azido peptides using click chemistry. The solid-phase peptide synthesis (SPPS) allows preparing the peptide dendron in high purity and establishing the possibility of automation. The presented peptide dendron is a promising candidate as multivalent ligand and was used for antibody B13-DE1 recognition. The binding affinity increases with higher dendron generation without loss of specificity. The analysis of biospecific interaction between the synthesized peptide dendron and the antibody was done via surface plasmon resonance (SPR) technique. The presented results show a promising tool for investigations of antigen-antibody reactions.
KW  - click chemistry
KW  - lysine dendron
KW  - peptide mimotopes
KW  - solid-phase peptide synthesis
KW  - surface plasmon resonance
Y1  - 2015
U6  - https://doi.org/10.1111/cbdd.12444
SN  - 1747-0277
SN  - 1747-0285
VL  - 85
IS  - 5
SP  - 565
EP  - 573
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Hovestaedt, Marc
A1  - Memczak, Henry
A1  - Pleiner, Dennis
A1  - Zhang, Xin
A1  - Rappich, Joerg
A1  - Bier, Frank Fabian
A1  - Stöcklein, Walter F. M.
T1  - Characterization of a new maleimido functionalization of gold for surface plasmon resonance spectroscopy
JF  - Journal of molecular recognition : an international journal devoted to research on specific molecular recognition in chemistry, biology, biotechnology and medicine
N2  - Para-maleimidophenyl (p-MP) modified gold surfaces have been prepared by one-step electrochemical deposition and used in surface plasmon resonance (SPR) studies. Therefore, a FITC mimotope peptide (MP1, 12 aa), a human mucin 1 epitope peptide (MUC, 9 aa) and a protein with their specific antibodies were used as model systems. The peptides were modified with an N-terminal cysteine for covalent and directed coupling to the maleimido functionalized surface by means of Michael addition. The coupling yield of the peptide, the binding characteristics of antibody and the unspecific adsorption of the analytes were investigated. The results expand the spectrum of biosensors usable with p-MP by widely used SPR and support its potential to be versatile for several electrochemical and optical biosensors. This allows the combination of an electrochemical and optical read-out for a broad variety of biomolecular interactions on the same chip. Copyright (c) 2014 John Wiley & Sons, Ltd.
KW  - biosensor
KW  - surface plasmon resonance
KW  - diazonium coupling
KW  - maleimidophenyl
KW  - cys-peptide
KW  - aryl diazonium salts
Y1  - 2014
U6  - https://doi.org/10.1002/jmr.2396
SN  - 0952-3499
SN  - 1099-1352
VL  - 27
IS  - 12
SP  - 707
EP  - 713
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -