TY  - JOUR
A1  - Laux, Eva-Maria
A1  - Bier, Frank Fabian
A1  - Hölzel, Ralph
T1  - Dielectrophoretic Stretching of DNA
JF  - DNA Nanotechnology
N2  - The spatial control of DNA and of self-assembled DNA constructs is a prerequisite for the preparation of DNA-based nanostructures and microstructures and a useful tool for studies on single DNA molecules. Here we describe a protocol for the accumulation of dissolved lambda-DNA molecules between planar microelectrodes by the action of inhomogeneous radiofrequency electric fields. The resulting AC electrokinetic forces stretch the DNA molecules and align them parallel to the electric field. The electrode preparation from off-the-shelf electronic components is explained, and a detailed description of the electronic setup is given. The experimental procedure is controlled in real-time by fluorescence microscopy.
KW  - Alignment
KW  - Dielectrophoresis
KW  - DNA
KW  - Electrokinetics
KW  - Interdigitated electrodes
KW  - Stretching
Y1  - 2018
SN  - 978-1-4939-8582-1
SN  - 978-1-4939-8581-4
U6  - https://doi.org/10.1007/978-1-4939-8582-1_14
SN  - 1064-3745
SN  - 1940-6029
SP  - 199
EP  - 208
PB  - Humana Press Inc.
CY  - New York
ET  - 2
ER  - 
TY  - JOUR
A1  - Stanke, Sandra
A1  - Bier, Frank Fabian
A1  - Hoezel, Ralph
T1  - Fluid streaming above interdigitated electrodes in dielectrophoresis experiments
JF  - Electrophoresis : microfluidics, nanoanalysis & proteomics
N2  - For the investigation of alternating current electrokinetic effects, a system is presented that allows for the simultaneous observation of fluid flow above and around microelectrodes in all three directions in space. Beside the usual microscopical view from top, lateral observation through the same objective is made possible by two small mirrors that are placed next to the electrodes. Fluid flow and movement of fluorescent nanoparticles above interdigitated electrodes are monitored by fluorescence microscopy and digital imaging and are further analysed by image processing. Field frequencies are varied from 10 Hz to 1 GHz at up to 10V(rms). Electrical conductivity of the fluid is monitored in situ in the actual measuring chamber.
KW  - Dielectrophoresis
KW  - Fluid streaming
KW  - Nanobead
Y1  - 2011
U6  - https://doi.org/10.1002/elps.201100096
SN  - 0173-0835
VL  - 32
IS  - 18
SP  - 2448
EP  - 2455
PB  - Wiley-Blackwell
CY  - Malden
ER  -