TY  - JOUR
A1  - Kamranfar, Iman
A1  - Xue, Gang-Ping
A1  - Tohge, Takayuki
A1  - Sedaghatmehr, Mastoureh
A1  - Fernie, Alisdair R.
A1  - Balazadeh, Salma
A1  - Mueller-Roeber, Bernd
T1  - Transcription factor RD26 is a key regulator of metabolic reprogramming during dark-induced senescence
JF  - New phytologist : international journal of plant science
N2  - Leaf senescence is a key process in plants that culminates in the degradation of cellular constituents and massive reprogramming of metabolism for the recovery of nutrients from aged leaves for their reuse in newly developing sinks. We used molecular-biological and metabolomics approaches to identify NAC transcription factor (TF) RD26 as an important regulator of metabolic reprogramming in Arabidopsis thaliana. RD26 directly activates CHLOROPLAST VESICULATION (CV), encoding a protein crucial for chloroplast protein degradation, concomitant with an enhanced protein loss in RD26 over-expressors during senescence, but a reduced decline of protein in rd26 knockout mutants. RD26 also directly activates LKR/SDH involved in lysine catabolism, and PES1 important for phytol degradation. Metabolic profiling revealed reduced c-aminobutyric acid (GABA) in RD26 overexpressors, accompanied by the induction of respective catabolic genes. Degradation of lysine, phytol and GABA is instrumental for maintaining mitochondrial respiration in carbon-limiting conditions during senescence. RD26 also supports the degradation of starch and the accumulation of mono-and disaccharides during senescence by directly enhancing the expression of AMY1, SFP1 and SWEET15 involved in carbohydrate metabolism and transport. Collectively, during senescence RD26 acts by controlling the expression of genes across the entire spectrum of the cellular degradation hierarchy.
KW  - Arabidopsis
KW  - fatty acid
KW  - primary metabolism
KW  - protein and amino acid degradation
KW  - respiration
KW  - senescence
Y1  - 2018
U6  - https://doi.org/10.1111/nph.15127
SN  - 0028-646X
SN  - 1469-8137
VL  - 218
IS  - 4
SP  - 1543
EP  - 1557
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Lisso, Janina
A1  - Altmann, Thomas
A1  - Müssig, Carsten
T1  - Metabolic changes in fruits of the tomato d(x) mutant
JF  - Phytochemistry : an international journal of plant biochemistry
KW  - Solanum lycopersicum
KW  - Solanaceae
KW  - tomato
KW  - brassinosteroid
KW  - primary metabolism
KW  - fruit
Y1  - 2006
U6  - https://doi.org/10.1016/j.phytochem.2006.07.008
SN  - 0031-9422
VL  - 67
IS  - 20
SP  - 2232
EP  - 2238
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Ribeiro, Dimas M.
A1  - Araujo, Wagner L.
A1  - Fernie, Alisdair R.
A1  - Schippers, Jos H. M.
A1  - Müller-Röber, Bernd
T1  - Translatome and metabolome effects triggered by gibberellins during rosette growth in Arabidopsis
JF  - Journal of experimental botany
N2  - Although gibberellins (GAs) are well known for their growth control function, little is known about their effects on primary metabolism. Here the modulation of gene expression and metabolic adjustment in response to changes in plant (Arabidopsis thaliana) growth imposed on varying the gibberellin regime were evaluated. Polysomal mRNA populations were profiled following treatment of plants with paclobutrazol (PAC), an inhibitor of GA biosynthesis, and gibberellic acid (GA(3)) to monitor translational regulation of mRNAs globally. Gibberellin levels did not affect levels of carbohydrates in plants treated with PAC and/or GA(3). However, the tricarboxylic acid cycle intermediates malate and fumarate, two alternative carbon storage molecules, accumulated upon PAC treatment. Moreover, an increase in nitrate and in the levels of the amino acids was observed in plants grown under a low GA regime. Only minor changes in amino acid levels were detected in plants treated with GA(3) alone, or PAC plus GA(3). Comparison of the molecular changes at the transcript and metabolite levels demonstrated that a low GA level mainly affects growth by uncoupling growth from carbon availability. These observations, together with the translatome changes, reveal an interaction between energy metabolism and GA-mediated control of growth to coordinate cell wall extension, secondary metabolism, and lipid metabolism.
KW  - Gibberellin
KW  - growth
KW  - paclobutrazol
KW  - primary metabolism
KW  - translatome
Y1  - 2012
U6  - https://doi.org/10.1093/jxb/err463
SN  - 0022-0957
VL  - 63
IS  - 7
SP  - 2769
EP  - 2786
PB  - Oxford Univ. Press
CY  - Oxford
ER  -