TY  - JOUR
A1  - Malinova, Irina
A1  - Kössler, Stella
A1  - Orawetz, Tom
A1  - Matthes, Ulrike
A1  - Orzechowski, Slawomir
A1  - Koch, Anke
A1  - Fettke, Jörg
T1  - Identification of two Arabidopsis thaliana plasma membrane transporters able to transport glucose 1-phosphate
JF  - Plant & cell physiology
N2  - Primary carbohydrate metabolism in plants includes several sugar and sugar-derivative transport processes. Over recent years, evidences have shown that in starch-related transport processes, in addition to glucose 6-phosphate, maltose, glucose and triose-phosphates, glucose 1-phosphate also plays a role and thereby increases the possible fluxes of sugar metabolites in planta. In this study, we report the characterization of two highly similar transporters, At1g34020 and At4g09810, in Arabidopsis thaliana, which allow the import of glucose 1-phosphate through the plasma membrane. Both transporters were expressed in yeast and were biochemically analyzed to reveal an antiport of glucose 1-phosphate/phosphate. Furthermore, we showed that the apoplast of Arabidopsis leaves contained glucose 1-phosphate and that the corresponding mutant of these transporters had higher glucose 1-phosphate amounts in the apoplast and alterations in starch and starch-related metabolism.
KW  - apoplast
KW  - Arabidopsis thaliana
KW  - glucose 1-phosphate transport
KW  - starch metabolism
KW  - sugar transport
Y1  - 2020
U6  - https://doi.org/10.1093/pcp/pcz206
SN  - 0032-0781
SN  - 1471-9053
VL  - 61
IS  - 2
SP  - 381
EP  - 392
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Lisso, Janina
A1  - Schröder, Florian
A1  - Müssig, Carsten
T1  - EXO modifies sucrose and trehalose responses and connects the extracellular carbon status to growth
JF  - Frontiers in plant science
N2  - Plants have the capacity to adapt growth to changing environmental conditions. This implies the modulation of metabolism according to the availability of carbon (C). Particular interest in the response to the C availability is based on the increasing atmospheric levels of CO2. Several regulatory pathways that link the C status to growth have emerged. The extracellular EXO protein is essential for cell expansion and promotes shoot and root growth. Homologous proteins were identified in evolutionarily distant green plants. We show here that the EXO protein connects growth with C responses. The exo mutant displayed altered responses to exogenous sucrose supplemented to the growth medium. Impaired growth of the mutant in synthetic medium was associated with the accumulation of starch and anthocyanins, altered expression of sugar-responsive genes, and increased abscisic acid levels. Thus, EXO modulates several responses related to the C availability. Growth retardation on medium supplemented with 2-deoxy-glucose, mannose, and palatinose was similar to the wildtype. Trehalose feeding stimulated root growth and shoot biomass production of exoplants where as it inhibited growth of the wildtype. The phenotypic features of the exo mutant suggest that apoplastic processes coordinate growth and C responses.
KW  - EXO
KW  - growth
KW  - sugar response
KW  - trehalose
KW  - apoplast
Y1  - 2013
U6  - https://doi.org/10.3389/fpls.2013.00219
SN  - 1664-462X
VL  - 4
IS  - 25
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  -