TY  - JOUR
A1  - Steppert, Isabel
A1  - Schönfelder, Jessy
A1  - Schultz, Carolyn
A1  - Kuhlmeier, Dirk
T1  - Rapid in vitro differentiation of bacteria by ion mobility spectrometry
JF  - Applied Microbiology and Biotechnology
N2  - Rapid screening of infected people plays a crucial role in interrupting infection chains. However, the current methods for identification of bacteria are very tedious and labor intense. Fast on-site screening for pathogens based on volatile organic compounds (VOCs) by ion mobility spectrometry (IMS) could help to differentiate between healthy and potentially infected subjects. As a first step towards this, the feasibility of differentiating between seven different bacteria including resistant strains was assessed using IMS coupled to multicapillary columns (MCC-IMS). The headspace above bacterial cultures was directly drawn and analyzed by MCC-IMS after 90 min of incubation. A cluster analysis software and statistical methods were applied to select discriminative VOC clusters. As a result, 63 VOC clusters were identified, enabling the differentiation between all investigated bacterial strains using canonical discriminant analysis. These 63 clusters were reduced to 7 discriminative VOC clusters by constructing a hierarchical classification tree. Using this tree, all bacteria including resistant strains could be classified with an AUC of 1.0 by receiver-operating characteristic analysis. In conclusion, MCC-IMS is able to differentiate the tested bacterial species, even the non-resistant and their corresponding resistant strains, based on VOC patterns after 90 min of cultivation. Although this result is very promising, in vivo studies need to be performed to investigate if this technology is able to also classify clinical samples. With a short analysis time of 5 min, MCC-IMS is quite attractive for a rapid screening for possible infections in various locations from hospitals to airports. Key Points center dot Differentiation of bacteria by MCC-IMS is shown after 90-min cultivation. center dot Non-resistant and resistant strains can be distinguished. center dot Classification of bacteria is possible based on metabolic features.
KW  - Bacteria identification
KW  - Volatile organic compounds (VOC)
KW  - Ion mobility
KW  - spectrometry (IMS)
KW  - Antibiotic resistance
KW  - Infection
KW  - Diagnostic
Y1  - 2021
U6  - https://doi.org/10.1007/s00253-021-11315-w
SN  - 0175-7598
SN  - 1432-0614
VL  - 105
IS  - 10
SP  - 4297
EP  - 4307
PB  - Springer
CY  - New York
ER  - 
TY  - JOUR
A1  - Kruckenberg, Helmut
A1  - Müller, Thomas
A1  - Freuling, Conrad
A1  - Mühle, Ralf-Udo
A1  - Globig, Anja
A1  - Schirrmeier, Horst
A1  - Buss, Melanie
A1  - Harder, Timm
A1  - Kramer, Matthias
A1  - Teske, Kathrin
A1  - Polderdijk, Kees
A1  - Wallschläger, Hans-Dieter
A1  - Hlinak, Andreas
T1  - Serological and virological survey and resighting of marked wild geese in Germany
JF  - European journal of wildlife research
N2  - In order to investigate the potential role of arctic geese in the epidemiology, the spatial and temporal spread of selected avian diseases, in autumn 2002, a virological and serological survey designed as capture-mark-resighting study was conducted in one of the most important coastal resting sites for migratory waterfowl in Germany. Orophatyngeal, cloacal swabs and blood samples were collected from a total of 147 birds comprising of three different arctic geese species including White-fronted Goose (Anser albifrons), Tundra Bean Goose (Anser fabalis rossicus), Pink-footed Goose (Anser brachyrhynchus) as well as from 29 non-migratory Canada Geese (Branta canadensis). Altogether, six adeno-like viruses (ALV; 95% CI, 1.74-9.92%) and two avian paramyxoviruses (APMV-4; 95% Cl, 0.19-5.53%) were isolated mainly from juvenile White-fronted Geese. In addition, four Canada Geese were infected with lentogenic APMV-1 (95% CI, 3.89-31.66%) at the date of sampling. No avian influenza viruses, reo-like viruses could be isolated despite serological evidence. Likewise, no evidence of current or previous infection by West Nile virus was found. Of the 147 birds tagged in the following years, 137 birds were resighted between 2002 and 2008 accumulating to 1925 sightings. About 90% of all sightings were reported from the main wintering and resting sites in Germany and The Netherlands. Eight of the resighted geese were virus positive (ALV and APMV-4) at the time point of sampling in 2002.
KW  - Wild geese
KW  - Anser albifrons
KW  - White-fronted goose
KW  - Disease
KW  - Spatial distribution
KW  - Winter
KW  - Distribution
KW  - Branta canadensis
KW  - Canada goose
KW  - Virus
KW  - Infection
KW  - Neckbanding
Y1  - 2011
U6  - https://doi.org/10.1007/s10344-011-0514-1
SN  - 1612-4642
VL  - 57
IS  - 5
SP  - 1025
EP  - 1032
PB  - Springer
CY  - New York
ER  -