TY - JOUR A1 - Gietler, Marta A1 - Nykiel, Malgorzata A1 - Orzechowski, Slawomir A1 - Fettke, Jörg A1 - Zagdanska, Barbara T1 - Protein carbonylation linked to wheat seedling tolerance to water deficiency JF - Environmental and experimental botany N2 - The appearance of the first leaf from the coleoptile in wheat seedlings (Triticum aestivum L.) coincides with the development of seedling susceptibility to water deficiency on the fifth day following imbibition. In dehydrated wheat seedlings, an increase in the protein carbonyl group has been observed. The coincidence of higher protein carbonylation levels with development of dehydration intolerance drew our attention. To gain more insight into the molecular basis of wheat drought tolerance, the seedling profiles of carbonylated proteins were analysed and compared. Two-dimensional gel electrophoresis (2D-PAGE) and mass spectrometry (MALDI-TOF and LC-MS/MS) were used to indicate and identify differential carbonylated proteins. Among the protein spots with at least a two-fold change in protein abundance in dehydrated seedlings in relation to control (well-watered) plants during the tolerant phase of growth, 19 carbonylated proteins increased and 18 carbonylated proteins decreased in abundance. Among 26 differentially expressed carbonylated proteins in sensitive seedlings, the abundance of 10 protein spots increased while that of 16 proteins decreased upon dehydration. We have demonstrated a link between protein carbonylation and seedling sensitivity to dehydration. The analysis of carbonylated protein profiles clearly showed that proteins with a potential role in the maintenance of dehydration tolerance in wheat seedlings are mainly linked to energy production, anti-fungal and/or insecticidal activity, or to the regulation of both protein synthesis and degradation. KW - Protein carbonylation KW - Dehydration tolerance KW - Triticum aestivum L. KW - Seedlings KW - Proteomic Y1 - 2017 U6 - https://doi.org/10.1016/j.envexpbot.2017.02.004 SN - 0098-8472 SN - 1873-7307 VL - 137 SP - 84 EP - 95 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Gietler, Marta A1 - Nykiel, Malgorzata A1 - Orzechowski, Slawomir A1 - Zagdanska, Barbara A1 - Fettke, Jörg T1 - Proteomic analysis of S-nitrosylated and S-glutathionylated proteins in wheat seedlings with different dehydration tolerances JF - Plant physiology and biochemistry : an official journal of the Federation of European Societies of Plant Physiology N2 - A loss of dehydration tolerance in wheat seedlings on the fifth day following imbibition is associated with a disturbance in cellular redox homeostasis, as documented by a shift of the reduced/oxidized glutathione ratio to a more oxidized state and a significant increase in the ratio of protein thiols to the total thiol group content. Therefore, the identification and characterization of redox-sensitive proteins are important steps toward understanding the molecular mechanisms of the loss of dehydration tolerance. In the present study, proteins that were differentially expressed between fully turgid (control), dehydrated tolerant (four-day-old) and dehydrated sensitive (six-day-old) wheat seedlings were analysed. Protein spots having at least a significant (p < 0.05) two-fold change in protein abundance were selected by Delta2D as differentially expressed, identified by MALDI-TOF and LC-MS/MS, and classified according to their function. The observed changes in the proteomic patterns of the differentially S-nitrosylated and S-glutathionylated proteins were highly specific in dehydration-tolerant and-sensitive wheat seedlings. The metabolic function of these proteins indicates that dehydration tolerance is mainly related to nucleic acids, protein metabolism, and energy metabolism. It has been proven that leaf-specific thionins BTH6 and DB4, chloroplastic 50S ribosomal protein L16, phospholipase A1-II delta, and chloroplastic thioredoxin M2 are both S-nitrosylated and S-glutathionylated upon water deficiency. Our results revealed the existence of interplay between S-nitrosylation and S-glutathionylation, two redox-regulated protein posttranslational modifications that could enhance plant defence mechanisms and/or facilitate the acclimation of plants to unfavourable environmental conditions. (C) 2016 Elsevier Masson SAS. All rights reserved. KW - Dehydration tolerance KW - Proteomics KW - Redox sensitive proteins KW - S-glutathionylation KW - S-nitrosylation KW - Triticum aestivum L. Y1 - 2016 U6 - https://doi.org/10.1016/j.plaphy.2016.08.017 SN - 0981-9428 VL - 108 SP - 507 EP - 518 PB - Elsevier CY - Paris ER -